Journal ArticleDOI
Monoclonal antibody Ki-67: its use in histopathology.
D. C. Brown,K C Gatter +1 more
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TLDR
Monoclonal antibody Ki‐67 is of use in research, providing a means of measuring proliferative activity in a variety of conditions besides malignancy, and may prove of value in monitoring tumour response to established and trial therapies.Abstract:
Monoclonal antibody Ki-67 is a reliable and easy means of accurately assessing the growth fraction of human neoplasms. Although the number of long-term follow-up studies is limited, it does appear to provide valuable prognostic information particularly in lymphoproliferative disease. Since the estimation of growth fraction is only one factor influencing tumour behaviour it would be naive to believe that measurement of this parameter alone, no matter how accurately, would provide the clinician with definitive prognostic information for all tumours. The antibody is also of use in research, providing a means of measuring proliferative activity in a variety of conditions besides malignancy, and may prove of value in monitoring tumour response to established and trial therapies.read more
Citations
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The cell proliferation-associated antigen of antibody Ki-67: a very large, ubiquitous nuclear protein with numerous repeated elements, representing a new kind of cell cycle-maintaining proteins.
TL;DR: Computer analysis of the nucleic acid and the deduced amino acid sequence of the Ki-67 antigen confirmed that the cDNA encodes for a nuclear and short-lived protein without any significant homology to known sequences.
Journal ArticleDOI
Chronic stage multiple sclerosis lesions contain a relatively quiescent population of oligodendrocyte precursor cells.
TL;DR: The identification of ways of stimulating the endogenous oligodendrocyte precursor population to expand and generate remyelinating cells may represent an alternative to transplantation of oligodendedrocytes lineage cells to promote myelin repair in MS.
Journal ArticleDOI
Ki67 protein: the immaculate deception?
D.C. Brown,K C Gatter +1 more
TL;DR: In this paper, the authors provide an update on the characterization of the Ki67 protein, its function and its use as a prognostic or diagnostic tool, as well as the number of papers published featuring this antibody has increased 10-fold from 338 to 3489.
Journal ArticleDOI
Proliferative Activity and Invasiveness among Pituitary Adenomas and Carcinomas: An Analysis Using the MIB-1 Antibody
Kamal Thapar,K. Kovacs,Bernd W. Scheithauer,Lucia Stefaneanu,Eva Horvath,Peter J. Pernicone,David Murray,Edward R. Laws +7 more
TL;DR: Although invasive pituitary tumors exhibited significantly higher growth fractions than did noninvasive tumors, there were individual exceptions, indicating that in a subpopulation of invasive pituitsary tumors, factors other than proliferative activity determine invasive potential.
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Reactive Astrocytes Form Scar-Like Perivascular Barriers to Leukocytes during Adaptive Immune Inflammation of the CNS
Rhonda R. Voskuhl,R. Scott Peterson,Bingbing Song,Yan Ao,Laurie Beth J. Morales,Seema K. Tiwari-Woodruff,Michael V. Sofroniew +6 more
TL;DR: Experimental evidence is provided that reactive astrocytes form scar-like perivascular barriers that restrict the influx of leukocytes into CNS parenchyma and protect CNS function during peripherally initiated, acquired immune inflammatory responses in the CNS.
References
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Journal Article
Cell cycle analysis of a cell proliferation-associated human nuclear antigen defined by the monoclonal antibody Ki-67.
TL;DR: The data suggest that the early stages of mitogen stimulation represent initial sequences of proliferation and not parts of the cell cycle, and immunostaining with monoclonal antibody Ki-67 provides a reliable means of rapidly evaluating the growth fraction of normal and neoplastic human cell populations.
Journal ArticleDOI
Production of a mouse monoclonal antibody reactive with a human nuclear antigen associated with cell proliferation
TL;DR: A first series of immunostainings of tumour biopsies indicated that Ki‐67 may be a potent tool for easy and quick evaluation of the proportion of proliferating cells in a tumour.
Journal ArticleDOI
Soft‐tissue sarcomas of adults; study of pathological prognostic variables and definition of a histopathological grading system
Monique Trojani,Geneviève Contesso,Jean Michel Coindre,Jacques Rouëssé,Nguyen Binh Bui,A. De Mascarel,J. F. Goussot,Marc David,Françoise Bonichon,Lagarde C +9 more
TL;DR: A second multivariate analysis introducing clinical prognostic features showed that the histological grade was the most important prognostic factor for soft‐tissue sarcomas and appears to be highly interesting because of its prognostic value and the facility of its elaboration.
Journal ArticleDOI
Increased expression of mutant forms of p53 oncogene in primary lung cancer.
TL;DR: Mutation of the p53 gene is the most frequently identified genetic change in human lung cancer; these findings suggest that simple immunohistological methods can provide strong evidence of such mutation.
Journal Article
Analysis of proliferative grade using anti-PCNA/cyclin monoclonal antibodies in fixed, embedded tissues. Comparison with flow cytometric analysis.
TL;DR: Semiquantitative immunocytochemical analysis with anti-PCNA/cyclin antibodies may represent a simple, reproducible, yet powerful technique for the routine analysis of cell kinetic data in alcohol-fixed, paraffin-embedded tissue by the surgical pathologist.