Journal ArticleDOI
Cell mediated immune responses to plaque antigens during experimental gingivitis in man.
TLDR
Results suggest that A. visocus is capable of inducing a specific cellular immune response during the development of experimental gingivitis and may be important as a causative plaque constituent.Abstract:
The purpose of the present investigation was to evaluate the ability of antigens of human dental plaque microorganisms to stimulate peripheral blood lymphocytes (PBL) from humans during the development and recovery from experimental gingivitis.
Eleven healthy dental students (22–36 years) participated in the study. At the start of the experiment, they exhibited clean teeth and clinically healthy gingivae. They then abstained from all oral hygiene measures for four weeks according to the model proposed by Loe et al. (1965). At the start, after 1, 2, 3 and 4 weeks of abolished oral hygience and 2 weeks following reinstitution of oral hygiene, the oral conditions were assessed, using the critieria of the Plaque Index system (Loe & Silness 1964) and the Gingival Index system (Loe & Silness (1963).
At each examination triplicate microcultures, each containing 2 × 105 viable PBL in .2 ml TC 199 with glutamine and antibiotics containing 10% fetal calf serum, were stimulated with four concentrations of sonicates from V. alcalescens, F. nucleatum, B. melaninogenicus, A. viscosus, A. naeslundii, S. sanguis and pooled dental plaque all obtained from the previsouly performed experimental gingivitis. The cultures were incubated for 78 hours at 37°C in 5% CO2. 3H-thymidine was added for the final 8 hours.
Only A. viscosus was able to stimulate PBL to undergo blastogenesis during the development of experimental gingivitis. A. viscosus stimulated the PBL of 3 subjects after one week but all subjects after 2 weeks of abolished oral hygiene. The stimulation indices (SI) remained >2.5 for the rest of the experimental period. Following 2 weeks of reinstituted oral hygiene, the SI reached preexperimental levels.
These results suggest that A. visocus is capable of inducing a specific cellular immune response during the development of experimental gingivitis and may be important as a causative plaque constituent. Further research is indicated to study this association.read more
Citations
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Journal ArticleDOI
Subgingival microflora and periodontal disease
TL;DR: The available data on oral microbial ecology suggest that the presence of dental plaque containing Gram-positive organisms may be essential for the attachment and colonization of several Gram-negative species after their initial introduction into the mouth and the periodontal pocket area.
Journal ArticleDOI
Clinical and microbiological effects of subgingival restorations with overhanging or clinically perfect margins
TL;DR: Changes in the subgingival microflora after the placement of restorations with overhanging margins document a potential mechanism for the initiation of periodontal disease associated with iatrogenic factors.
Journal ArticleDOI
Bacteriology of human experimental gingivitis: effect of plaque and gingivitis score.
W. J. Loesche,Salam A. Syed +1 more
TL;DR: The bacteriological profile showed that when bleeding occurred, the levels and proportions of A. viscosus and B. melaninogenicus increased significantly, and the possibility that proportional changes in the gingival plaque flora may uniquely contribute to the development of gingivitis is raised.
Journal ArticleDOI
Microbial etiology of periodontal disease
TL;DR: Evidence supports the concept that the etiology of gingivitis is bacteriologically nonspecific and future research on the bacteriology of periodontal disease should concentrate on longitudinal studies; particular attention should be paid to the change of gedivitis into periodontitis whereby animals as well as humans can be used.
Journal ArticleDOI
Label-free quantitative proteomics reveals differentially regulated proteins in experimental gingivitis
Nagihan Bostanci,Per Ramberg,Asa Wahlander,Jonas Grossman,Daniel Jönsson,Virginia Barnes,Panos N. Papapanou +6 more
TL;DR: The data demonstrate that LC-MS/MS label-free quantitative proteomics is valuable in the assessment of the protein content of the GCF and can facilitate a better understanding of the molecular mechanisms involved in the induction and resolution of plaque-induced gingival inflammation in humans.
References
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Journal ArticleDOI
Periodontal Disease in Pregnancy II. Correlation Between Oral Hygiene and Periodontal Condition
John Silness,Harald Löe +1 more
TL;DR: In this paper, the correlation between oral hygiene and periodontal condition was investigated in the context of pregnant women with Periodontal Disease in Pregnancy II (PDI II).
Journal ArticleDOI
Periodontal disease in pregnancy. i. prevalence and severity.
Harald Löe,John Silness +1 more
TL;DR: (1963).
Journal ArticleDOI
Stimulation of lymphocyte transformation by bacterial antigens in patients with periodontal disease
L. Ivanyi,Thomas Lehner +1 more
TL;DR: The cell-mediated immune response to some oral microorganisms may play a protective or aggressive part in the pathogenesis of periodontal disease.
Journal ArticleDOI
Subgingival microflora and periodontitis.
TL;DR: Predominant bacterial flora resident in subgingival plaque was characterized and evaluated in relation to the etiology of periodontal disease and no statistically significant differences were observed in the flora between clinically normal and pathological sites of the same patients.
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