Citric Acid Cycle: Gene-Enzyme Relationships in Bacillus subtilis
Blanka Rutberg,James A. Hoch +1 more
TLDR
An effect of mutation conferring loss of one enzyme of the cycle on the specific activity of the other enzymes in the cycle was observed.Abstract:
The genetic location of mutations affecting the citric acid cycle and the properties of mutants of Bacillus subtilis possessing these mutations have been examined. Genes coding for the component enzymes of the cycle were found to be unlinked to each other and thus do not form an operon. The mutational defect in a mutant lacking fumarase mapped between thr-5 and cysB3. Mutations causing inability to produce isocitrate dehydrogenase and succinate dehydrogenase were found to map between argA11 and leu-1. The alpha-ketoglutarate dehydrogenase mutations were mapped at the terminal end of the B. subtilis chromosome through a weak linkage in phage PBS-1 transduction of one class of these mutations of ilvA2 and metB4. A second class of alpha-ketoglutarate dehydrogenase mutations mapped closer to ilvA2 and metB4 but still terminal with respect to these markers. Aconitaseless mutants possessed mutations that could not be linked to any of the known transducing segments of the chromosome. An effect of mutation conferring loss of one enzyme of the cycle on the specific activity of the other enzymes in the cycle was observed.read more
Citations
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Role of glutamate metabolism in bacterial responses towards acid and other stresses
TL;DR: The role of glutamate metabolism in response against acid stress and other stresses is reviewed, and many bacteria are capable of metabolizing GABA to succinate via the GABA shunt pathway.
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Expression of competence genes in Bacillus subtilis.
TL;DR: A set of competence (com) mutants of Bacillus subtilis was constructed by using Tn917lacZ as a mutagen and it is concluded that expression of com genes is probably regulated transcriptionally and in a growth stage-specific manner.
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Revised genetic linkage map of Bacillus subtilis
Patrick J. Piggot,James A. Hoch +1 more
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The Bacillus subtilis chromosome.
Dennisj . Henner,James A. Hoch +1 more
TL;DR: A comparison of the B. SUBTILIS and ESCHERICHIA COLI GENETIC MAPS shows that the former is superior to the latter in terms of properties and the latter is inferior on the basis of size and application.
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