Journal ArticleDOI
Classification of Embryogenic Cell-lines of Picea abies as Regards Protoplast Isolation and Culture
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TLDR
Embryogenic cell-lines of Picea abies were divided into two groups based on their growth habit and the morphology of the somatic embryos, with the highest proportion of dividing cells entering the embryogenic pathway obtained from cell- lines belonging to group A cultured on solid medium.About:
This article is published in Journal of Plant Physiology.The article was published on 1993-02-01. It has received 43 citations till now. The article focuses on the topics: Somatic embryogenesis & Protoplast.read more
Citations
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Embryogeny of gymnosperms: advances in synthetic seed technology of conifers
S. M. Attree,Larry C. Fowke +1 more
TL;DR: This review focuses on recent advances in conifer somatic embryogenesis and synthetic seed technology, particularly in areas of embryo development, maturation drying, encapsulation and germination.
Journal ArticleDOI
Evolution of the PEBP Gene Family in Plants: Functional Diversification in Seed Plant Evolution
Anna Karlgren,Niclas Gyllenstrand,Thomas Källman,Jens F. Sundström,David P. Moore,Martin Lascoux,Ulf Lagercrantz +6 more
TL;DR: A first duplication event resulting in two families of plant PEBP genes (MFT-like and FT/TFL1-like) seems to coincide with the evolution of seed plants, in which independent control of bud and seed dormancy was required, and the second duplication resulting in the FT- like and TFL1 -like clades probably coincided with the Evolution of angiosperms.
Journal ArticleDOI
Developmental pathway of somatic embryogenesis in Picea abies as revealed by time-lapse tracking.
TL;DR: The lack of staining of the arabinogalactan protein epitope recognized by the monoclonal antibody JIM13 was shown to be an efficient marker for distinguishing proembryogenic masses from somatic embryos.
Journal ArticleDOI
Importance of arabinogalactan proteins for the development of somatic embryos of Norway spruce (Picea abies)
TL;DR: It is shown that extracellular proteins concentrated from group A cell lines can stimulate group B embryos to develop further and that seed extract can stably convert B embryos into A embryos and that development of somatic embryos in Norway spruce is associated with particularextracellular AGPs, which have a regulatory function.
Journal ArticleDOI
Somatic embryogenesis and somaclonal variation in Norway spruce: morphogenetic, cytogenetic and molecular approaches.
TL;DR: Four embryogenic clones of Norway spruce have been subcultivated and observed over several years to determine the evolution of production of mature embryos and to assess the quality of the embryos produced, revealing three cases of mutated acclimated somatic plants.
References
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Journal ArticleDOI
Nutritional Requirements for Growth of Vicia hajastana Cells and Protoplasts at a Very Low Population Density in Liquid Media
K. N. Kao,M. R. Michayluk +1 more
TL;DR: Vicia cells were able to grow in a mineral-salt solution supplemented with sucrose (or glucose), a few vitamins, and 2,4-dichlorophenoxyacetic acid but were not able to survive when cultured at a low population density unless the medium was supplemented with zeatin, naphthalene-1-acetic Acid, nucleic-acid bases, amino acids, other sugars, sugar alcohols, and organic acids.
Journal ArticleDOI
In vitro studies of adventitious shoot formation in Pinus contorta
Sara von Arnold,Tage Eriksson +1 more
TL;DR: Isolated embryos of Pinus contorta Dougl.
Journal ArticleDOI
Efficient Transformation of Arabidopsis-Thaliana Using Direct Gene-Transfer to Protoplasts
TL;DR: Transformation experiments performed with a selectable and a non-selectable gene on separate plasmids resulted in a co-transformation rate of functionally active copies in about 25% of the transformants analysed, suggesting this approach can be used to introduce non- selectable genes into the Arabidopsis genome.
Journal ArticleDOI
Improved Efficiency of Somatic Embryogenesis in Mature Embryos of Picea abies (L.) Karst.
TL;DR: Embryogenic callus was formed from mature zygotic embryos of Picea abies cultured in darkness on media containing 5 to 20 μM 2,4-D or NAA and 5 μM BA on the basis of sucrose concentration and proline addition up to 15 mM.
Conifer suspension culture medium development using analytical data from developing seeds
John D. Litvay,Morris A. Johnson,Devi C. Verma,Dean W. Einspahr,Hilkka M. Kaustinen,Keith Weyrauch +5 more
TL;DR: An initial attempt to correct this imbalance has resulted in a culture medium formulation which has allowed tissues from juvenile seedlings or mature trees of Douglas-fir and loblolly pine to be grown and maintained as fine cell suspensions for prolonged periods.
Related Papers (5)
Importance of arabinogalactan proteins for the development of somatic embryos of Norway spruce (Picea abies)
Improved Efficiency of Somatic Embryogenesis in Mature Embryos of Picea abies (L.) Karst.
In vitro studies of adventitious shoot formation in Pinus contorta
Sara von Arnold,Tage Eriksson +1 more