Color, phenolic and flavonoid content, and antioxidant activity of honey from Roraima, Brazil
Jonierison Alves Pontis,Luiz Antonio Mendonça Alves da Costa,Silvio José Reis da Silva,Adriana Flach +3 more
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TLDR
The total phenolic and flavonoid content, color, and antioxidant activity were evaluated from ten honey samples from Apis mellifera L. collected from Roraima State, Brazil as mentioned in this paper.Abstract:
The total phenolic and flavonoid content, color, and antioxidant activity were evaluated from ten honey samples from Apis mellifera L. collected from Roraima State, Brazil. The total phenolic content was determined using the Folin-Ciocalteu reagent and ranged from 250 to 548 mg gallic acid kg –1 of honey. The total flavonoid content was obtained using two methods: total flavones/flavonols were determined with aluminum chloride, and flavonones/dihydroflavonols were determined with 2,4-dinitrophenylhydrazine. The results ranged from 9 to 48.6 mg of quercetine kg –1 of honey and 1805 to 2606 mg of pinocembrin kg –1 of honey, respectively. Antioxidant activity was measured with 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavengers, and the results of the IC 50 ranged from 3.17 to 8.79 mg/mL. Statistical analysis demonstrated positive correlations between color intensity, flavone and flavonol content, and phenolic content and negative correlations with antioxidant capacity.read more
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References
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Standardization of antioxidant properties of honey by a combination of spectrophotometric/fluorimetric assays and chemometrics
TL;DR: In this article, the authors established a solid platform of analytical information for the definition/standardization of the antioxidant properties of honey, using a battery of spectrophotometric tests: Folin-Ciocalteu assay for phenol content, ferric reducing antioxidant power (FRAP) for total antioxidant activity, 1,1-diphenyl-2-picrylhydrazyl (DPPH) assay for antiradical activity, absorbance:450 (ABS 450 ) for color intensity and one fluorimetric method: ORAC,
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Antioxidant activity of Portuguese honey samples: Different contributions of the entire honey and phenolic extract
TL;DR: In this paper, the antioxidant activity of Portuguese honeys was evaluated considering the different contribution of entire samples and phenolic extracts and a discriminant analysis was also performed, giving satisfactory results once the six samples were clustered in six individual groups obtained through the definition of two discriminant analyses dimensions.
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Advances in the analysis of phenolic compounds in products derived from bees.
Ana María Gómez-Caravaca,María Gómez-Romero,David Arráez-Román,Antonio Segura-Carretero,Alberto Fernández-Gutiérrez +4 more
TL;DR: An overview of current analytical methods for measuring polyphenols in honey and propolis is presented, based on spectrophotometry as well as analytical separation techniques such as gas chromatography, high-pressure liquid chromatography and capillary electrophoresis.
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Radical scavenging activity of different floral origin honey and beebread phenolic extracts
TL;DR: In this article, a preliminary screening of phenolic compounds in honey samples was performed by high-performance liquid chromatography with UV and mass spectrometer detectors, and the results obtained showed that all samples contain p-coumaric acid, kaempferol, chrysin and apigenin.
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Validated methods for the quantification of biologically active constituents of poplar-type propolis
Milena Popova,Vassya Bankova,Daniela Butovska,Valentin Petkov,Boryana Nikolova-Damyanova,Anna Gloria Sabatini,Gian Luigi Marcazzan,Stefan Bogdanov +7 more
TL;DR: The validation of rapid, low-cost spectrophotometric procedures for the quantification of the three main groups of bioactive substances in poplar-type propolis has been performed and two sets of results agreed satisfactory, as proven by Student's t-test.