Comparison of enumeration techniques for the investigation of bacterial pollution in the Berg River, Western Cape, South Africa
TLDR
The study was aimed at assessing techniques, which would provide an accurate indication of the planktonic bacterial pollution load in the Berg River, Western Cape, South Africa Sampling of sites started in June 2004 and continued for a period of 1 year until June 2005.Abstract:
The study was aimed at assessing techniques, which would provide an accurate indication of the planktonic bacterial pollution load in the Berg River, Western Cape, South Africa Sampling of sites started in June 2004 and continued for a period of 1 year until June 2005 The most probable number (MPN) technique was used to determine the level of faecal coliforms and E coli, while the heterotrophic plate count method was used to determine the amount of culturable micro-organisms in planktonic samples The flow cytometry (FCM) and direct acridine orange count (DAOC) (epifluorescence microscopy) techniques were employed to evaluate total bacterial counts in planktonic (water) samples The highest MPN and heterotrophic plate counts were recorded in Week 37 at site B2 at 17 x 107 micro-organisms/100 m and 104 x 106micro- organisms/m, respectively In comparison, the viable FCM counts, were significantly higher (p < 005) for that period at 17 x 107 micro-organisms/m The highest total FCM count of 37 x 107 micro-organisms/m was recorded in Week 41 at Site B2 In comparison the highest DAOC of 83 x 106 micro-organisms/m was obtained in Week 29 at Site B2 Results showed that on average the heterotrophic plate count represented a fraction (< 365%) of the total FCM counts The total DAOC count also represented a fraction (< 4308%) of the total FCM count for most of the sampling period Results therefore showed that the FCM proved to be more effective in evaluating microbial pollution in water samplesread more
Citations
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Tracking composition and dynamics of nitrification and denitrification microbial community in a biofilm reactor by PCR-DGGE and combining FISH with flow cytometry
TL;DR: Nested analysis of variance assay (ANOVA) demonstrated that the C/N ratio did not significantly affect the shift of populations in different groups of nitrifying and denitrifying bacteria detected by FISH-FCM.
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The deteriorating nutrient status of the Berg River, South Africa
TL;DR: In this paper, the authors investigated the nutrient status of the Berg River and long-term trends therein, showing that inorganic nitrogen and phosphorus levels increase downstream by a factor of more than 10, in response to anthropogenic inputs.
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Comparison of microbial contamination at various sites along the Plankenburg: and Diep Rivers, Western Cape , South Africa
TL;DR: In this paper, the authors investigated and compared the microbial contamination levels at various sites in the Plankenburg and Diep Rivers in the Western Cape, South Africa, by means of the most probable number (MPN) technique, the number of culturable cells were determined using the heterotrophic plate count (HPC) technique and total microbial counts were evaluated by Flow cytometric analysis.
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Co-detection of virulent Escherichia coli genes in surface water sources.
TL;DR: The McNemar's test and the Pearson Chi-square were used to assess the co-detection and observed frequency, respectively, for potentially virulent E. coli genes in river water and while early detection is crucial, quantitative microbial risk analysis has to be performed to identify and estimate the risk to human health.
Journal ArticleDOI
Urbanisation, climate change and its impact on water quality and economic risks in a water scarce and rapidly urbanising catchment: case study of the Berg River catchment
James D. S. Cullis,Annabel Horn,Nico Rossouw,L. Fisher-Jeffes,Marlé M. Kunneke,Willem Hoffman +5 more
TL;DR: In this paper, the authors explored the increasing water quality risks due to climate change and rapid urban development and the likely direct and indirect economic impacts that this will have on the agriculture sector, which is a key contributor to the regional and national economy.
References
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Standard Methods for the Examination of Water and Wastewater seventh edition
TL;DR: Standard Methods for the Examination of Water and Wastewater seventh edition, Standard methods for the examination of water and wastewater seventh edition , کتابخانه دیجیتال جندی اهواز.
Journal ArticleDOI
Phylogenetic identification and in situ detection of individual microbial cells without cultivation.
TL;DR: Phylogenetic analysis of the retrieved rRNA sequence of an uncultured microorganism reveals its closest culturable relatives and may, together with information on the physicochemical conditions of its natural habitat, facilitate more directed cultivation attempts.
UseofNuclepore Filters forCounting Bacteria by Fluorescence Microscopy
TL;DR: Polycarbonate Nuclepore filters are better than cellulose filters for the direct counting of bacteria because they have uniform pore size and a flat surface that retains all of the bacteria on top of the filter.
Journal ArticleDOI
Use of nuclepore filters for counting bacteria by fluorescence microscopy.
TL;DR: Polycarbonate Nuclepore filters are better than cellulose filters for the direct counting of bacteria because they have uniform pore size and a flat surface that retains all of the bacteria on top of the filter.
Book
Clinical Microbiology Procedures Handbook
TL;DR: This well-respected reference continues to serve as the sole major publication providing step-by-step descriptions that enable clinical microbiologists and their staffs to perform all analyses and their control from the receipt of the specimen to the final report.