Journal ArticleDOI
Computer simulation for the prediction of separation as a function of pH for reversed-phase high-performance liquid chromatography. II. Resolution as a function of simultaneous change in pH and solvent strength.
TLDR
The optimization of reversed-phase high-performance liquid chromatographic separation by the simultaneous variation of pH and solvent strength (%B) was studied for acidic and basic samples and the combination of these two variables was expected to be more useful than either variable alone.About:
This article is published in Journal of Chromatography A.The article was published on 1992-02-21. It has received 69 citations till now. The article focuses on the topics: High-performance liquid chromatography & Benzoic acid.read more
Citations
More filters
Journal ArticleDOI
Retention behaviour of quinolone derivatives in high-performance liquid chromatography. Effect of pH and evaluation of ionization constants.
TL;DR: In this paper, the effect of solute ionization on the retention behavior of a series of quinolones and evaluates their pKa values using chromatographic data in acetonitrile-water mixtures.
Journal ArticleDOI
Changing reversed-phase high performance liquid chromatography selectivity Which variables should be tried first?
TL;DR: This study provides a quantitative evaluation of different variables for their effect on selectivity (alpha) and various practical problems which must be balanced against this ability of a variable to change value of alpha.
Journal ArticleDOI
The application of factor analysis to solvatochromic parameters and pHs values for the standardization of potentiometric sensors in mobile phases used in liquid chromatography
TL;DR: In this paper, the pH s values for reference buffer solutions in aqueous acetonitrile, methanol, and tetrahydrofuran mixtures used as mobile phases in LC were subjected to factor analysis in order to discover the number of factors which affect the variation with the percentage of organic co-solvent of the whole data sets.
Journal ArticleDOI
Retention of ionisable compounds on high-performance liquid chromatography. XV. Estimation of the pH variation of aqueous buffers with the change of the acetonitrile fraction of the mobile phase
TL;DR: From both the estimated pH values of the mobile phase and the estimated pKa of the ionisable analytes, it is possible to predict the degree of ionisation of the analytes and therefore, the interpretation of acid-base analytes behaviour in a particular methanol-water buffered mobile phase.
Journal ArticleDOI
Artificial Neural Network in Drug Delivery and Pharmaceutical Research
TL;DR: Artificial neural networks do not require rigidly structured experimental designs and can map functions using historical or incomplete data, which makes them a powerful tool for simulation of various non-linear systems.
References
More filters
Journal ArticleDOI
Gradient selection in reversed-phase liquid chromatography
TL;DR: In this article, a general expression is derived for the numerical calculation of retention data for the case when the relationship between the capacity factor,k, and the mobile phase composition, φ, is known.
Journal ArticleDOI
Influence of organic modifiers on the rentention behaviour in reversed-phase liquid chromatography and its consequences for gradient elution
TL;DR: In this paper, an extensive study of 32 aromatic compounds in aqueous system containing 10-100% of methanol, acetonitrile and tetrahydro-furan as organic modifiers under isocratic conditions is described.
Journal ArticleDOI
DryLab computer simulation for high-performance liquid chromatographic method development. II. Gradient elution.
TL;DR: In this paper, computer simulation as an aid for the development of gradient high-performance liquid chromatographic methods is reviewed and several examples of its application are presented and the accuracy of such predictions is discussed.
Journal ArticleDOI
B and C terms in the Van Deemter equation for liquid chromatography
John H. Knox,Hugh P. Scott +1 more
TL;DR: In this article, the authors derived explicit equations for the B and C terms in the Van Deemter equation for liquid chromatography, involving diffusion coefficients in the mobile zone, the stagnant mobile phase and the stationary phase.
Journal ArticleDOI
Drylab® computer simulation for high-performance liquid chromatographic method development
TL;DR: Computer simulation (DryLab software) as an aid for the development of gradient high-performance liquid chromatographic methods is reviewed and the accuracy of such predictions is discussed.