Journal ArticleDOI
Construction of large-volume monolithic columns.
TLDR
A heat release during the polymerization is analyzed and a mathematical model is derived for the prediction of the maximal thickness of the monolithic annulus having a uniform structure for GMA-EDMA monoliths.Abstract:
Monolithic supports have become the subject of extensive study in the past years. Despite their advantageous features and many successful chromatographic applications in the analytical scale, only a very few examples of larger volume monoliths were described. In the case of GMA−EDMA monoliths, this can be attributed to the fact that due to the exothermic polymerization a pronounced temperature increase inside the monolith significantly affects the structure. The temperature increase depends on the thickness of the monolith, and consequently, there is an upper limit that allows the preparation of a unit with a uniform structure. In the present work, we have analyzed a heat release during the polymerization and have derived a mathematical model for the prediction of the maximal thickness of the monolithic annulus having a uniform structure. On the basis of the calculations, two annuluses of different diameters were polymerized and merged into a single monolithic unit with a volume of 80 mL. In addition, a s...read more
Citations
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Journal ArticleDOI
Applications of silica-based monolithic HPLC columns
TL;DR: An overview covering various aspects of this new column type in the field of high throughput analysis of drugs and metabolites, chiral separations, analysis of pollutants and food-relevant compounds, as well as in bioanalytical separations such as in proteomics is given.
Journal ArticleDOI
Monolithic stationary phases for liquid chromatography and capillary electrochromatography.
TL;DR: A monolithic stationary phase is the continuous unitary porous structure prepared by in situ polymerization or consolidation inside the column tubing and the surface is functionalized to convert it into a sorbent with the desired chromatographic binding properties.
Journal ArticleDOI
Silica gel-based monoliths prepared by the sol-gel method: facts and figures.
TL;DR: The purpose of this paper is to deal with the problems associated with silica based monoliths, including cracking and the shrinkage of the bed during drying.
Journal ArticleDOI
Chromatographic media for bioseparation
TL;DR: In this review, the action principles, features of chromatography media regarding physical and chemical properties will be described and an attempt will be made to establish categories of different media.
Journal ArticleDOI
Polymeric monolithic materials : Syntheses, properties, functionalization and applications
TL;DR: In this article, the synthesis of polymer-based monolithic materials is discussed, focusing on the aspects of controlling pore sizes, pore volumes and pore size distributions as well as functionalization of these supports.
References
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Journal ArticleDOI
Continuous rods of macroporous polymer as high-performance liquid chromatography separation media
Journal ArticleDOI
High-performance liquid chromatography on continuous polymer beds
TL;DR: The experiments described in refs.
Journal ArticleDOI
Phase Separation in Gelling Silica–Organic Polymer Solution: Systems Containing Poly(sodium styrenesulfonate)
Kazuki Nakanishi,Naohiro Soga +1 more
TL;DR: In this article, phase separation in parallel to the hydrolysis and gelation of alkoxysilane solution containing poly(sodium styrenesulfonate) (NaPSS) has been investigated.
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High-Performance Membrane Chromatography. A Novel Method of Protein Separation
TL;DR: In this article, 1 mm thick membranes from macroporous methacrylate polymer provided with functional groups were synthetized and used for protein separation and the results show that the separation is fully comparable with that experienced on a filled column but the advantage of a membrane is up to two orders of magnitude lower pressure during the process and very high loading reaching up to 40 g/m2.