Journal ArticleDOI
Cybernetic modeling of bacterial cultures at low growth rates: mixed-substrate systems
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TLDR
In this paper, a cybernetic model was proposed to predict the low growth rate behavior of bacteria in mixed-substrate environment using only growth and maintenance parameters from single substrate experiments, which accurately predicts the simultaneous substrate utilization and maintenance energy effects in constant fed-batch cultures of Klebsiella oxytoca.Abstract:
A cybernetic model to predict the low-growth-rate behavior of bacteria in mixed-substrate environment is presented. Using only growth and maintenance parameters from single-substrate experiments, the model accurately predicts the simultaneous substrate utilization and maintenance energy effects in constant fed-batch cultures of Klebsiella oxytoca. The robustness of the model was examined more rigorously by perturbing glucose-limited fed-batch cultures with additions of arabinose, xylose, and fructose. In all cases, reasonable agreement of the model prediction with the experimental data was observed.read more
Citations
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Journal ArticleDOI
Growth Kinetics of Suspended Microbial Cells: From Single-Substrate-Controlled Growth to Mixed-Substrate Kinetics
Karin Kovárová-Kovar,Thomas Egli +1 more
TL;DR: The data suggest that a dilemma exists, namely, that either “intrinsic” KS or μmax can be measured but both cannot be determined at the same time, which should result in a competitive advantage of a cell capable of mixed-substrate growth because it can grow much faster at low substrate concentrations than one would expect from single- substrate kinetics.
Journal ArticleDOI
Modelling of microbial kinetics
Jens Nielsen,John Villadsen +1 more
TL;DR: This review of bacterial and fungal models is preceded by a discussion of a general framework for the study of microbial kinetics and the use of a two-dimensional discretization of the kinetic model wherein both the individual cell and the cell mass is structured is shown to be helpful in dealing with comlex fermentation systems.
Journal ArticleDOI
Microbial physiology and ecology of slow growth.
TL;DR: Earlier theories for the kinetics of uptake through the cell envelope and steady-state systems of metabolic enzymes are discussed and a computer simulation is presented, and the problems to the cell of fluctuating levels of nutrient and too much substrate during continuous culture are discussed.
Journal ArticleDOI
Understanding and prediction of soil microbial community dynamics under global change
TL;DR: In this article, a review of soil microbial processes in northern terrestrial ecosystems and predictions of their possible modifications under anticipated global changes is presented, where the main focus is the dependence of population dynamics of soil microorganisms on environmental factors.
Journal ArticleDOI
Mathematical modelling of ethanol production from glucose/xylose mixtures by recombinant Zymomonas mobilis
TL;DR: A two-substrate model based on substrate limitation, substrate inhibition, and product (ethanol) inhibition was evaluated, and it was established that the model provides good predictions of experimental batch culture data for 25/25, 50/50, and 65/65 g l−1 glucose/xylose media.
References
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Journal ArticleDOI
The Maintenance Energy of Bacteria in Growing Cultures
TL;DR: From the laws of growth, a simple relation between the maintenance requirement, the growth yield and the growth rate is derived and is shown to be in good agreement with the available data.
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Maintenance energy: a general model for energy-limited and energy-sufficient growth.
TL;DR: Consistent values of the maximum growth yield can be derived, irrespective of whether the cultures are energy limited or energy sufficient, and the possibility that the constant maintenance energy term may be estimated from the maximum specific growth rate is considered.
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The status of YATP and maintenance energy as biologically interpretable phenomena.
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Strategies of mixed substrate utilization in microorganisms
Wim Harder,Lubbert Dijkhuizen +1 more
TL;DR: Recent studies on mixed substrate utilization in both batch and continuous cultures have thrown light on the strategies of the control mechanisms that, in microbes, govern the utilization of the various substrates and indicated the possible significance of mixed substrates in microbial competition in nutrient-limited natural ecosystems.
Journal ArticleDOI
Investigation of bacterial growth on mixed substrates: experimental evaluation of cybernetic models
TL;DR: Cybernetic models, developed earlier by the authors, have been evaluated experimentally for the growth of Klebsiella oxytoca in batch cultures using mixed substrates from glucose, xylose, arabinose, lactose, and fructose, accurately predicting the order in which the substrates are consumed.