De novo design of helical bundles as models for understanding protein folding and function.
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Structural and mutational analysis allowed us to explore the determinants of native protein structure and these determinants were then applied to the design of a dinuclear metal-binding protein that can now serve as a model for this important class of proteins.Abstract:
De novo protein design has proven to be a powerful tool for understanding protein folding, structure, and function. In this Account, we highlight aspects of our research on the design of dimeric, four-helix bundles. Dimeric, four-helix bundles are found throughout nature, and the history of their design in our laboratory illustrates our hierarchic approach to protein design. This approach has been successfully applied to create a completely native-like protein. Structural and mutational analysis allowed us to explore the determinants of native protein structure. These determinants were then applied to the design of a dinuclear metal-binding protein that can now serve as a model for this important class of proteins.read more
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Supramolecular catalysis. Part 2: artificial enzyme mimics
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Design of folded peptides.
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Synthetic models for non-heme carboxylate-bridged diiron metalloproteins: strategies and tactics.
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The design of coiled-coil structures and assemblies
TL;DR: The current design rules for coiled coils are summarized, some of the key successful coiled-coil designs that have been created to date are described, and an important development in the field is shown; namely, new designs are being created with function as well as structure in mind.
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