Dengue Virus Plaque Formation on Microplate Cultures and Its Application to Virus Neutralization

TLDR: By use of the microplate cultures, in combination with the transfer-plates reported by previous investigators, dengue neutralization tests were performed and a sigmoid curve relationship was revealed between the decrease in virus titer and reciprocals of antiserum concentration.

Abstract: Discussion and SummaryDengue virus plaque formation on BHK-21 cell micro-plate cultures was described. The clear plaques were visible usually 5 days after incubation in a CO2 incubator at 37°. The cells cultured in a 3-oz bottle were sufficient to prepare two microculture plates which were usually ready for use after 1-2 days of cultivation in the CO2 incubator at 37°. The overall procedures were easy and of economic advantage.It is to be stated in this connection that the affinity of dengue viruses to tissue culture cells is not necessarily high, so that the cell culture systems suitable for dengue virus plaquing have so far been limited; and even in such suitable systems the formation of clear plaques takes a much longer time of incubation than for other kinds of arbo-viruses in general. Some of the difficulties regarding this matter have been overcome by the techniques reported here.By use of the microplate cultures, in combination with the transfer-plates reported by previous investigators (8), dengue...