Journal ArticleDOI
Determination of folates in foods by high-performance liquid chromatography with fluorescence detection after precolumn conversion to 5-methyltetrahydrofolates.
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TLDR
It has been possible to show that the deconjugation of the folates by rat plasma conjugase was incomplete in foodstuffs whereas chicken pancreas conjugases effectively converted the different folate polyglutamates into folate diglutamate.About:
This article is published in Journal of Chromatography A.The article was published on 2001-08-31. It has received 98 citations till now. The article focuses on the topics: Polyglutamate.read more
Citations
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Respective effects of milk composition and the cheese-making process on cheese compositional variability in components of nutritional interest
Anthony Lucas,Anthony Lucas,Edmond Rock,Jean-François Chamba,Isabelle Verdier-Metz,Patrick Brachet,Jean-Baptiste Coulon +6 more
TL;DR: In this article, the relation entre the conditions de production du lait and the teneurs en composes d'interet nutritionnel dans le fromage is discussed.
Journal ArticleDOI
Expression of prostate-specific membrane antigen (PSMA), increases cell folate uptake and proliferation and suggests a novel role for PSMA in the uptake of the non-polyglutamated folate, folic acid
TL;DR: This work examined if expression of human PSMA in PC‐3 cells confers a proliferative advantage in a microenvironment with physiologically relevant folate levels.
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The analysis of folate and its metabolic precursors in biological samples.
Journal ArticleDOI
HPLC determination of folates in raw and processed beetroots
TL;DR: In this paper, a sensitive HPLC method with fluorescence detection and gradient elution has been developed for the determination of folates in vegetables, which was applied to analysis of raw and processed beetroots; 5-methyltetrahydrofolate was found to be the main folate form in beetroots.
Analytical, Nutritional and Clinical Methods HPLC determination of folates in raw and processed beetroots
TL;DR: In this paper, a sensitive HPLC method with fluorescence detection and gradient elution has been developed for the determination of folates in vegetables, which was applied to analysis of raw and processed beetroots; 5-methyltetrahydrofolate was found to be the main folate form in beetroots.
References
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Journal ArticleDOI
Lactobacillus casei microbiological assay of folic acid derivatives in 96-well microtiter plates.
D W Horne,D Patterson +1 more
TL;DR: This procedure, used with modern, computer-interfaced microtiter-plate readers and data-reduction software, greatly shortens the time and minimizes reagent costs for this assay.
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Methods of vitamin assay
TL;DR: In this paper, a variety of methods for conducting vitamin assays are described, including biological assays, microbiological assays and chromatographic assays; these methods are also discussed in the context of specific vitamins and nutrients.
Journal ArticleDOI
Determination of Folate in Cereal-Grain Food Products Using Trienzyme Extraction and Combined Affinity and Reversed-Phase Liquid Chromatography
TL;DR: A trienzyme treatment (employing rat plasma folate conjugase, α-amylase, and protease), followed by affinity chromatography and reversed-phase HPLC, was evaluated for quantification of folates in u...
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A validated liquid chromatographic method for determining folates in vegetables, milk powder, liver, and flour.
TL;DR: Results for total folates were lower than results obtained with microbiological methods, but use of a commercially available folate-binding protein for cleanup saved time and money and was effective.
Journal ArticleDOI
The availability of food folate in man.
T. Tamura,E. L. R. Stokstad +1 more
TL;DR: The results indicated that pure synthetic folate derivatives are almost as available as PteGlu.