Journal ArticleDOI
Developmental changes in chromosomal composition and template activity during spermatogenesis in trout testis.
Keiji Marushige,Gordon H. Dixon +1 more
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Developmental changes in the composition of isolated chromatins and their ability to support RNA synthesis have been studied in trout testes undergoing spermatogenesis artificially induced by gonadotropin injections.About:
This article is published in Developmental Biology.The article was published on 1969-04-01. It has received 142 citations till now. The article focuses on the topics: Chromatin & Histone.read more
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Journal ArticleDOI
Studies of Nuclear Acidic Proteins EVIDENCE FOR THEIR PHOSPHORYLATION, TISSUE SPECIFICITY, SELECTIVE BINDING TO DEOXYRIBONUCLEIC ACID, AND STIMULATORY EFFECTS ON TRANSCRIPTION
TL;DR: Methods are described for the extraction, separation, and electrophoretic analysis of a class of acidic nuclear proteins from various tissues of the rat and relationships are observed between DNA binding and enhancement of RNA synthesis.
Book ChapterDOI
Vertebrate protamine genes and the histone-to-protamine replacement reaction.
Rafael Oliva,Gordon H. Dixon +1 more
TL;DR: The fact that distinctive amino acids, such as threonine or valine, present in chicken protamine polypeptides, are conserved in position in the amino acid sequence of quail only if the sequence redetermined by Oliva and Dixon is considered, makes the probability of different allelic variants in chicken much less likely.
Journal ArticleDOI
Progesterone-binding Components of Chick Oviduct III. CHROMATIN ACCEPTOR SITES
TL;DR: Under optimal conditions, the progesterone-oviduct receptor complex displays more extensive binding to oviduct chromatin than to the chromatins of chick spleen, heart, mature erythrocytes, or liver, which indicates that the target tissue chromatin may contain acceptor sites for the steroid hormone-receptor complex.
Book ChapterDOI
Remodeling of nucleoproteins during gametogenesis, fertilization, and early development.
TL;DR: This chapter reviews the transformation of nucleoprotein types during gametogenesis, fertilization, and early development, outlines chromatin structural changes that accompany these nucleop protein transitions, and discusses some functions of the nucleoproteins and histone variants associated with gamete, zygote, andEarly embryonic nuclei.
Journal ArticleDOI
Mouse sperm chromatin proteins: quantitative isolation and partial characterization
TL;DR: Conditions are described that permit the quantitative extraction of chromatin proteins from the epididymal sperm of the mouse and evidence is presented that suggests that this single band contains two protamine-like proteins.
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Journal Article
Protein Measurement with the Folin Phenol Reagent
TL;DR: Procedures are described for measuring protein in solution or after precipitation with acids or other agents, and for the determination of as little as 0.2 gamma of protein.
Journal ArticleDOI
A procedure for the isolation of deoxyribonucleic acid from micro-organisms
TL;DR: A method has been described for the isolation of DNA from micro-organisms which yields stable, biologically active, highly polymerized preparations relatively free from protein and RNA, and Representative samples have been characterized for their thermal stability and sedimentation behaviour.
Book ChapterDOI
Assay of inorganic phosphate, total phosphate and phosphatase
TL;DR: The method is about seven times as sensitive as the Fiske–SubbaRow procedure and involves less pipetting, but it is not very satisfactory for determining inorganic phosphate if labile phosphate esters are present in large excess.
Book
The nucleic acids
Erwin. Chargaff,J. N. Davidson +1 more
TL;DR: Nucleic Acid is a complex biomolecule that stores genetic information in the form of a code that is necessary for life.