Journal ArticleDOI
Developmental genetics of the esterase isozymes of Fundulus heteroclitus
Roger S. Holmes,Gregory S. Whitt +1 more
TLDR
The ontogeny of the esterase isozymes of the teleost, Fundulus heteroclitus, has been investigated and there appear to be at least 15 different esterases, which constitute 6–8 groups, each of which is probably encoded in one or more genetic loci.Abstract:
The ontogeny of the esterase isozymes of the teleost, Fundulus heteroclitus, has been investigated. One group of esterase isozymes is present at all stages of development, whereas other esterase isozymes only very gradually appear at later stages of development, or abruptly appear at such dramatic developmental events as hatching. The ontogeny of these isozyme patterns is interpreted as the expression of differential regulation of separate esterase genes. The general pattern of teleost esterase gene activation is similar to that reported for birds and mammals. Allelic variation was detected at two of the esterase loci. On the basis of electrophoretic mobility, substrate specificity, inhibitor specificity, genetic variation, and ontogeny of esterases, there appear to be at least 15 different esterase isozymes, which constitute 6–8 groups, each of which is probably encoded in one or more genetic loci.read more
Citations
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Journal ArticleDOI
Production of clones of homozygous diploid zebra fish ( Brachydanio rerio )
TL;DR: Clones of homozygous fish have been produced from individual homozygotes and associated genetic methods facilitate genetic analyses of this vertebrate.
Journal ArticleDOI
Characterization and assay conditions for Use of AChE activity from several marine species in pollution monitoring
TL;DR: In this paper, the authors investigate the effects of anticholinesterase compounds on plaice (Pleuronectes platessa) and common prawn (Palaemon serratus) which have high activity levels.
Journal ArticleDOI
Evolutionary adaptations of gene structure and expression in natural populations in relation to a changing environment: a multidisciplinary approach to address the million-year saga of a small fish.
TL;DR: It is shown that variation in the regulatory sequence of Ldh-B is responsible for the differences in transcription rate between populations and that the patterns of variation are inconsistent with a neutral model of molecular evolution.
Journal ArticleDOI
Mitochondrial‐dna restriction‐site polymorphisms in the teleost fundulus heteroclitus support secondary intergradation
TL;DR: Analysis of mitochondrial‐DNA restriction electromorphs helps resolve the issue of not being able to distinguish between primary and secondary intergradation models for Fundulus heteroclitus on the basis of allelic isozymes or morphological data.
Journal ArticleDOI
Evolution of patterns of differential gene expression: A comparison of the temporal and spatial patterns of lsozyme locus expression in two closely related fish species (northern largemouth bass, Micropterus salmoides salmoides, and Smallmouth Bass, Micropterus dolomieui)
TL;DR: The timing of the onset of developmental expression of 33 loci encoding various enzymes and isozymes was determined for two closely related species of fish, the northern largemouth bass and the smallmouth bass, M. dolomieui, consistent with the hypothesis that there is an increase in divergence of gene expression in species which are progressively more distantly related.
References
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Journal ArticleDOI
Multiple forms of enzymes: tissue, ontogenetic, and species specific patterns.
Clement L. Markert,Freddy Møller +1 more
TL;DR: 2 Cleveland, L. R., J. R. R, and W. Nutting, J. L. Zool.
Journal ArticleDOI
The ontogeny of isozyme patterns of lactate dehydrogenase in the mouse
TL;DR: The enzyme lactate dehydrogenase was shown to exist as five electrophoretically distinct molecular varieties, or isozymes, within the tissues of the mouse, and it was shown that LDH-5 is more abundant in tissues subject to relative anaerobiosis, and the isoz enzymes at the other end of the spectrum areMore abundant in highly oxygenated tissues.
Journal ArticleDOI
Multiple forms of esterase in vertebrate blood plasma
TL;DR: Evidence will be presented to show that each of the esterase types exists in multiple forms, that each animal species has its own typical set of plasma esterases, and that various forms of each ester enzyme type exist within the same species.
Journal ArticleDOI
The distribution of esterases in mouse tissues.
TL;DR: Fractionation of liver homogenates by centrifugation showed esterase to be concentrated primarily in the microsome fraction, and the pattern of esterases was very similar in each of the centrifugal fractions.
Journal ArticleDOI
Developmental genetics of the lactate dehydrogenase isozymes of fish
TL;DR: Three genes (A, B, and E) encoding lactate dehydrogenase (LDH) subunits are shown to be present in fish by: (1) molecular hybridization, (2) allelic variants at two loci, and (3) kinetic, physical, and immunochemical studies.
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