Dipole interactions in electrofusion. Contributions of membrane potential and effective dipole interaction pressures.
TLDR
The contributions of pulse-induced dipole-dipole interaction to the total pressure acting normal to the membranes of closely positioned pronase treated human erythrocytes during electrofusion was calculated.About:
This article is published in Biophysical Journal.The article was published on 1991-05-01 and is currently open access. It has received 35 citations till now. The article focuses on the topics: Dipole & Polarizability.read more
Citations
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Journal ArticleDOI
Electrokinetics in micro devices for biotechnology applications
TL;DR: The range of influence for electrokinetically manipulated biological objects in microdevices, which can lead to interesting applications in biotechnology are reviewed and examined.
Journal ArticleDOI
The effect of electrical deformation forces on the electropermeabilization of erythrocyte membranes in low- and high-conductivity media.
TL;DR: Calculations showed that (i) electric force precedes and accompanies membrane charging (up to the breakdown voltage) and that under low-conductivity conditions, the electric stretching force contributes significantly to the enlargement of ``electroleaks'' in the plasma membrane generated by electric breakdown.
Journal ArticleDOI
Reversible Electropermeabilization of Mammalian Cells by High-Intensity, Ultra-Short Pulses of Submicrosecond Duration
TL;DR: Pulse power systems used here appear to be a promising tool for the electropermeabilization of very small cells and vesicles (including intracellular organelles, liposomes, etc.).
Journal ArticleDOI
Cell electrofusion using nanosecond electric pulses
TL;DR: It is shown that ns pulses can induce selective electroporation of the contact areas between cells, regardless of the cell size, and it is expected that these pulses can improve fusion yields in electrofusion of cells with different size, such as myeloma cells and B lymphocytes in hybridoma technology.
Journal ArticleDOI
Studies of cell pellets: II. Osmotic properties, electroporation, and related phenomena: membrane interactions.
I.G. Abidor,L.H. Li,Sek Wen Hui +2 more
TL;DR: The intercellular or intermembrane distances between cells in a pellet, as a function of osmotic, colloidal-osmotic, and centrifugal pressures used to compress rabbit erythrocyte pellets, were deduced from the Rp measurement, offering a unique opportunity to measure the inter Membrane repulsive force in a disordered system including living cells.
References
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Book ChapterDOI
Electrical breakdown, electropermeabilization and electrofusion
TL;DR: The techniques for electrical breakdown, electropermeabilization and electrofusion could be an important tool in this process, since the high electrical fields occurring naturally in the membrane play an important role in the selective transport of substances across the membrane as well as in natural regulatory processes.
Journal ArticleDOI
Cell adhesion. Competition between nonspecific repulsion and specific bonding
TL;DR: A thermodynamic calculus for the modeling of cell adhesion is developed, able to compute the end results of competition between the formation of specific macromolecular bridges and nonspecific repulsion arising from electrostatic forces and osmotic (steric stabilization) forces.
Journal ArticleDOI
Voltage-induced pore formation and hemolysis of human erythrocytes.
Kazuhiko Kinosita,Tian Yow Tsong +1 more
TL;DR: Isotonic suspensions of human erythrocytes were exposed to single electric pulses of intensity at a few kV/cm and duration in microseconds, and the cell membranes became permeable to Na+ and K+ and hemolysed through the colloid osmotic effect of hemoglobin.
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Electroporation of cell membrane visualized under a pulsed-laser fluorescence microscope.
Kazuhiko Kinosita,Ikuo Ashikawa,N. Saita,Hideyuki Yoshimura,Hiroyasu Itoh,K. Nagayama,Akira Ikegami +6 more
TL;DR: By constructing a fluorescence microimaging system with a submicrosecond time resolution, this work has been able to resolve temporally and spatially the events in a single cell under a microsecond electric pulse.
Journal ArticleDOI
Electro-mechanical permeabilization of lipid vesicles. Role of membrane tension and compressibility.
David Needham,Robert M. Hochmuth +1 more
TL;DR: The model predicts a relation between tension and voltage at breakdown and this relation is verified experimentally for the three reconstituted membrane systems studied here.
Related Papers (5)
Kinetics of ultrastructural changes during electrically induced fusion of human erythrocytes.
D. A. Stenger,S.W. Hui +1 more
Electro-mechanical permeabilization of lipid vesicles. Role of membrane tension and compressibility.
David Needham,Robert M. Hochmuth +1 more