Diversity and phylogenetic analysis of bacteria in the mucosa of chicken ceca and comparison with bacteria in the cecal lumen
Jianhua Gong,Robert J. Forster,Hai Yu,James R. Chambers,Parviz M. Sabour,Roger Wheatcroft,Shu Chen +6 more
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TLDR
Bacteria in the mucosa were highly diverse but mainly Gram-positive with low G+C and Fusobacterium prausnitzii and butyrate-producing bacteria comprised the largest groups among 116 cloned sequences.Abstract:
We reported the first attempt to describe mucosa-associated bacterial populations in the chicken ceca by molecular analysis of 16S rRNA genes. Bacteria in the mucosa were highly diverse but mainly Gram-positive with low G+C. Fusobacterium prausnitzii and butyrate-producing bacteria comprised the largest groups among 116 cloned sequences. Twenty five percent of the clones had less than 95% homology to database sequences. Many sequences were related to those of uncultured bacteria identified in human feces or the bovine rumen. Terminal restriction fragment length polymorphism (T-RFLP) analysis revealed some differences between bacterial populations present in the mucosa and lumen of ceca. Greater resolution of bacterial population was obtained using a culture-independent approach rather than a culture-based approach.read more
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Application of prebiotics and probiotics in poultry production
TL;DR: The intestinal microbiota, epithelium, and immune system provide resistance to enteric pathogens, and recent data suggest that resistance is not solely due to the sum of the components, but that cross-talk between these components is also involved in modulating this resistance.
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Diversity and Succession of the Intestinal Bacterial Community of the Maturing Broiler Chicken
Jiangrang Lu,Umelaalim Idris,Barry G. Harmon,Charles L. Hofacre,John J. Maurer,Margie D. Lee +5 more
TL;DR: The diversity of bacterial floras in the ilea and ceca of chickens that were fed a vegetarian corn-soy broiler diet devoid of feed additives was examined by analysis of 1,230 partial 16S rRNA gene sequences, suggesting that each region developed its own unique bacterial community as the bird matured.
Journal ArticleDOI
Molecular ecological analysis of the gastrointestinal microbiota: a review.
TL;DR: A critical summary is provided of current molecular ecological approaches for studying the GI microbiota, such as those based on functional genes and their expression and the combined use of stable isotopes and biomarkers.
References
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