Effect of GCSB-5, a Herbal Formulation, on Monosodium Iodoacetate-Induced Osteoarthritis in Rats.
Joon-Ki Kim,Sang-Won Park,Jung-Woo Kang,Yu-Jin Kim,Sung Youl Lee,Joon-Shik Shin,Sang-Ho Lee,Sun-Mee Lee +7 more
Reads0
Chats0
TLDR
Results indicate that GCSB-5 is a potential therapeutic agent for the protection of articular cartilage against progression of osteoarthritis through inhibition of MMPs activity, inflammatory mediators, and NF-κB activation.Abstract:
Therapeutic effects of GCSB-5 on osteoarthritis were measured by the amount of glycosaminoglycan in rabbit articular cartilage explants in vitro, in experimental osteoarthritis induced by intra-articular injection of monoiodoacetate in rats in vivo. GCSB-5 was orally administered for 28 days. In vitro, GCSB-5 inhibited proteoglycan degradation. GCSB-5 significantly suppressed the histological changes in monoiodoacetate-induced osteoarthritis. Matrix metalloproteinase (MMP) activity, as well as, the levels of serum tumor necrosis factor-α, cyclooxygenase-2, inducible nitric oxide synthase protein, and mRNA expressions were attenuated by GCSB-5, whereas the level of interleukin-10 was potentiated. By GCSB-5, the level of nuclear factor-κB p65 protein expression was significantly attenuated but, on the other hand, the level of inhibitor of κB-α protein expression was increased. These results indicate that GCSB-5 is a potential therapeutic agent for the protection of articular cartilage against progression of osteoarthritis through inhibition of MMPs activity, inflammatory mediators, and NF-κB activation.read more
Citations
More filters
Stimulation of cytokine and chemokine expression by human articular chondrocytes in response to fibronectin fragments
TL;DR: In this article, the authors used a 110kD FN-f (0.5-1µM) to stimulate chondrocyte expression of the pro-inflammatory cytokines IL-1, IL-6, IL -8, MCP-1 and GRO-s.
Journal ArticleDOI
Coupling Freshly Isolated CD44+ Infrapatellar Fat Pad‐Derived Stromal Cells with a TGF‐β3 Eluting Cartilage ECM‐Derived Scaffold as a Single‐Stage Strategy for Promoting Chondrogenesis
Henrique V. Almeida,Gráinne M. Cunniffe,Tatiana Vinardell,Conor T. Buckley,Fergal J. O'Brien,Fergal J. O'Brien,Daniel J. Kelly +6 more
TL;DR: A cartilage extracellular matrix (ECM)‐derived scaffold that could facilitate the rapid proliferation and chondrogenic differentiation of freshly isolated stromal cells is developed and opens up new possibilities for in‐theatre cell‐based therapies for joint regeneration.
Journal ArticleDOI
Usage report of pharmacopuncture in musculoskeletal patients visiting Korean medicine hospitals and clinics in Korea.
Yoon Jae Lee,Joon-Shik Shin,Jinho Lee,Me-riong Kim,Ki Byung Park,Hwa Dong Lee,Yoonmi Lee,Jungwan Hong,In-Hyuk Ha +8 more
TL;DR: Patterns of pharmacopuncture use for musculoskeletal disease treatment in Korea are verified, and use of Pharmacopuncture varied depending on disease or symptom severity, which is expected to contribute to future clinical study design and standardization.
Journal ArticleDOI
A prospective, randomized, double-blind, multicenter comparative study on the safety and efficacy of Celecoxib and GCSB-5, dried extracts of six herbs, for the treatment of osteoarthritis of knee joint
Yong-Geun Park,Chul-Won Ha,Chang Dong Han,Seong-Il Bin,Hee-Chun Kim,Young-Bok Jung,Hong-Chul Lim +6 more
TL;DR: The result of this study supports that GCSB-5 is comparable to Celecoxib in terms of the efficacy and safety for the treatment of osteoarthritis of knee joint.
Journal ArticleDOI
Effects of intra-articular SHINBARO treatment on monosodium iodoacetate-induced osteoarthritis in rats
Won Kyung Kim,Hwa Jin Chung,Yuna Pyee,Tae Jun Choi,Hyen Joo Park,Ji-Young Hong,Joon-Shik Shin,Jinho Lee,In-Hyuk Ha,Sang Kook Lee +9 more
TL;DR: Intra-articular administration of SHINBARO (IAS) at 20 mg/kg remarkably restrained the decrease in bone volume/total volume and modulated the balance of inflammatory enzymes, mediators, and cytokines in the MIA-induced osteoarthritis rat model.
References
More filters
Journal ArticleDOI
Interleukin-10 and the interleukin-10 receptor.
TL;DR: Findings that have advanced the understanding of IL-10 and its receptor are highlighted, as well as its in vivo function in health and disease.
Journal ArticleDOI
Gastrointestinal Toxicity With Celecoxib vs Nonsteroidal Anti-inflammatory Drugs for Osteoarthritis and Rheumatoid Arthritis: The CLASS Study: A Randomized Controlled Trial
Fred E. Silverstein,Gerald A. Faich,Jay L. Goldstein,Lee S. Simon,Theodore Pincus,Andrew Whelton,Robert W. Makuch,Glenn M. Eisen,Naurang M. Agrawal,William F. Stenson,Aimee M. Burr,William W. Zhao,Jeffrey D. Kent,James B. Lefkowith,Kenneth M. Verburg,G. Steven Geis +15 more
TL;DR: In this study, celecoxib, at dosages greater than those indicated clinically, was associated with a lower incidence of symptomatic ulcers and ulcer complications combined, as well as other clinically important toxic effects, compared with NSAIDs at standard dosages.
Journal ArticleDOI
Series Introduction: The transcription factor NF-κB and human disease
TL;DR: It is shown that the transcription factor NF-κB has been shown to be the target of several anti-inflammatory and anticancer drugs.
Journal ArticleDOI
The matrix metalloproteinases and their inhibitors.
TL;DR: A number of metalloproteinases that degrade the extracellular matrix of connective tissues and two specific tissue inhibitors of met ALLPs have now been isolated, characterized, and cloned, and initial studies have been carried out to assess the contribution to their biochemical and biologic properties.
Journal ArticleDOI
Weight bearing as a measure of disease progression and efficacy of anti-inflammatory compounds in a model of monosodium iodoacetate-induced osteoarthritis
Susan E. Bove,Susan L. Calcaterra,Rachel M. Brooker,C.M Huber,Roberto E. Guzman,P.L. Juneau,D.J Schrier,Kenneth S. Kilgore +7 more
TL;DR: The determination of differences in hind paw weight distribution in the rat MIA model of OA is a technically straightforward, reproducible method that is predictive of the effects of anti-inflammatory and analgesic agents and useful for the discovery of novel pharmacologic agents in human OA.