Effect of Inhibin on Testosterone Metabolism by Rat Ventral Prostate in Vitro
TLDR
Inhibin can modulate the conversion of testosterone to 5 alpha-DHT in the rat ventral prostate, and therefore can autocrine-paracrine function can be attributed to the prostate.Abstract:
Conversion of radiolabeled testosterone to 5 α-dihydrotestosterone (5 α-DHT) was determined in the rat ventral prostate in vitro, and the effect of inhibin on this conversion was demonstrated. The data revealed that the reduction of testosterone to 5 α-DHT was inhibited in a dose-related manner in the presence of inhibin as compared with the basal reduction. The percentage of testosterone reduced per 10-mg tissue also showed a similar trend. The ratio of radioactive counts for DHT and testosterone was also minimum at the highest does of inhibin used. Inhibin can thus modulate the conversion of testosterone to 5 α-DHT in the rat ventral prostate, and therefore can autocrine-paracrine function can be attributed to the prostate.read more
Citations
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Detection of prostatic-inhibin-like peptide in the cytoplasm of LNCaP cells, a human prostatic adenocarcinoma cell line
TL;DR: The presence of PIP is demonstrated in a human prostatic cell line, i.e., LNCaP cells, and has a close similarity with PIP isolated from the human seminal plasma.
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Immunocytochemical localization of bioregulatory peptides in marmoset testes.
TL;DR: In vitro biosynthesis of inhibin and FSH was increased by hCG, whereas prolactin and prostatic inhibin peptide suppressed the synthesis of these hormones.
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Modulation of rat prostatic inhibinlike peptide (PIP) by steroids and protein hormones.
TL;DR: Positive regulation of PIP by testosterone and stimulatory effect of estradiol-17 beta on PIP in rat ventral prostrate are demonstrated.
References
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Journal ArticleDOI
Endogenous steroid levels in the human prostate from birth to old age: a comparison of normal and diseased tissues.
TL;DR: After oestrogen treatment, testosterone and androstenedione virtually disappeared, while reduced but significant concentrations of their metabolites remained in similar proportions to those observed in the untreated carcinomatous tissue.
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Inhibin: 1985 update on action and purification.
TL;DR: It is concluded that there are at least 2 classes of proteins with inhibin-like activity: a relatively large molecular weight material with apparent molecular mass between 40 and 70 kDa found in gonadal extracts and fluids, and a smaller material, with Molecular mass between 5 and 20 kDa, found in seminal plasma.
Journal Article
Isolation & characterization of inhibin from human seminal plasma.
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Evidence for the prostatic origin of immunoreactive inhibin-like material in human seminal plasma.
TL;DR: It is concluded that the bulk of the immunoreactive ILM present in the semen, blood and urine of men is not secreted by the testes, and the principal site of origin of this material appears to be the prostate.
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Testosterone metabolism: A necessary step for activity?
Renato Massa,Luciano Martini +1 more
TL;DR: It has been found that the prostate, the anterior pituitary, the submaxillary gland and the pancreas have a rather elevated 5 α-reductase activity and the activity of the 3α-hydroxysteroid-dehydrogenase was found to be totally unrelated to the 5α-reducing capacity of each structure.