Effect of the hypocotyl hook on greening in etiolated cucumber cotyledons.
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The data suggest the contribution of some factor or factors by the hypocotyl hook which reduce the lag phase during greening during cotyledons excised.Abstract:
Four-day-old etiolated cucumber cotyledons (Cucumis sativus, L.) were excised and allowed to green in white fluorescent light at 28 C. Cotyledons excised with a full hypocotyl hook exhibited a lag phase of 1 hour before entering the rapid greening phase, whereas cotyledons excised without any hypocotyl hook exhibited a lag phase of 6 hours. Cotyledons excised with varying lengths of hypocotyl hook accumulated chlorophyll roughly in proportion to the hook length. When cotyledons were excised with a full hook and were partially or totally shielded from light with aluminum foil, the samples with the hook covered accumulated more chlorophyll than the wholly exposed samples. The samples with the cotyledons covered showed no net accumulation of chlorophyll irrespective of hook's exposure to light. These data suggest the contribution of some factor or factors by the hypocotyl hook which reduce the lag phase during greening.read more
Citations
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Effects of Iron and Oxygen on Chlorophyll Biosynthesis : II. OBSERVATIONS ON THE BIOSYNTHETIC PATHWAY IN ISOLATED ETIOCHLOROPLASTS.
TL;DR: A comprehensive hypothesis for the role of O(2) and Fe in chlorophyll biosynthesis is formulated.
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The Biosynthesis of delta-Aminolevulinic Acid in Higher Plants: I. Accumulation of delta-Aminolevulinic Acid in Greening Plant Tissues.
TL;DR: The findings of this investigation are consistent with the hypothesis that delta-aminolevulinic acid is a chlorophyll precursor in higher plants, and that chlorophyLL biosynthesis is regulated at the level of the formation of delta-aminaolevulic acid.
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Chloroplast biogenesis: Biosynthesis and accumulation of Mg-protoporphyrin IX monoester and longer wavelength metalloporphyrins by greening cotyledons
TL;DR: The Chromatographic and spectral properties of these compounds suggested that they represent intermediates between Mg-protoporphyrin IX monomethyl ester and protochlorophyllide.
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Chlorophyll biosynthesis in a cell-free system from higher plants.
TL;DR: A crude homogenate obtained from greening cucumber cotyledons in tris-sucrose, containing coenzyme A, glutathione, potassium phosphate, pH 7.7, is able to incorporate 4-(14)C-delta-aminolevulinic acid into chlorophyll a and b in the presence of oxygen.
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In Vitro Synthesis of the Chlorophyll Isocyclic Ring Transformation of Magnesium-Protoporphyrin IX and Magnesium-Protoporphyrin IX Monomethyl Ester into Magnesium-2,4-Divinyl Pheoporphyrin A5
TL;DR: The conversion was insensitive to inhibitors of iron-sulfur and heme-containing proteins, to Cu chelators, H(2)O(2), and peroxide scavengers, however, the conversion was extremely sensitive to phenazine methosulfate, methylene blue, and methyl viologen.
References
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Copper enzymes in isolated chloroplasts. polyphenoloxidase in beta vulgaris
TL;DR: Evidence that a copper enzyme, polyphenoloxidase (otherwise known as tyrosinase or catecholase), is localized in the chloroplasts of spinach beet (chard), Beta vu?garis is presented.
Journal ArticleDOI
The Effect of Age and Various Chemicals on the Lag Phase of Chlorophyll Synthesis in Dark Grown Bean Seedlings
Edward C. Sisler,W. H. Klein +1 more
Journal ArticleDOI
Protochlorophyll Formation and Greening in Etiolated Barley Leaves
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