Journal ArticleDOI
Electron microscopic observations of the distribution of acidic anionic residues on hamster spermatozoa and eggs before and during fertilization.
Ryuzo Yanagimachi,Ryuzo Yanagimachi,Garth L. Nicolson,Garth L. Nicolson,Y. D. Noda,Y. D. Noda,M. Fujimoto,M. Fujimoto +7 more
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TLDR
Acidic anionic residues on the surfaces of the hamster eggs and spermatozoa were investigated using a colloidal iron hydroxide (CIH)-labeling technique, and suggests that the sperm and egg plasma membrane components intermix during gamete fusion.About:
This article is published in Journal of Ultrastructure Research.The article was published on 1973-05-01. It has received 68 citations till now. The article focuses on the topics: Sperm head plasma membrane & Sperm.read more
Citations
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Book ChapterDOI
Mechanisms of Fertilization in Mammals
TL;DR: This chapter will discuss how mammalian spermatozoa prepare themselves for fertilization and how the spermatozosa and eggs interact during fertilization.
Journal ArticleDOI
Structure and function of the zona pellucida: identification and characterization of the proteins of the mouse oocyte's zona pellucida.
TL;DR: Results of amino acid analysis and high-resolution two-dimensional electrophoresis of the individual proteins suggest that each protein represents a unique polypeptide chain in the zona pellucida.
Journal ArticleDOI
The Fluid—Mosaic Model of Membrane Structure: Still relevant to understanding the structure, function and dynamics of biological membranes after more than 40 years
TL;DR: In updated versions of the model more emphasis has been placed on the mosaic nature of the macrostructure of cellular membranes where many protein and lipid components are limited in their rotational and lateral motilities in the membrane plane, especially in their natural states.
Journal ArticleDOI
Ultrastructural localization of lectin-binding sites on the zonae pellucidae and plasma membranes of mammalian eggs.
TL;DR: The asymmetric density of RCAI receptors across the zona was confirmed by ferritin- RCAI and fluorescein-RCAI labeling of mechanically isolated zonae pellucidae, indicating that the RCAi-binding sites are more densely distributed in the exterior zona regions.
Journal ArticleDOI
Membrane differentiations in freeze-fractured mammalian sperm
Daniel S. Friend,Don W. Fawcett +1 more
TL;DR: A correlated thin-sectioning and freeze-fracturing study has been made of guinea pig and rat spermatozoa and observations form a basis for further studies on the functional correlates of these regional specializations of the sperm membrane.
References
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Journal ArticleDOI
The fluid mosaic model of the structure of cell membranes.
S. J. Singer,Garth L. Nicolson +1 more
TL;DR: Results strongly indicate that the bivalent antibodies produce an aggregation of the surface immunoglobulin molecules in the plane of the membrane, which can occur only if the immunoglOBulin molecules are free to diffuse in the membrane.
Journal ArticleDOI
The movement of golden hamster spermatozoa before and after capacitation.
TL;DR: The movement of these capacitated spermatozoa of the golden hamster is quite different from that of fresh epididymal (uncapacitated) spermatoza, which shows an extraordinarily active movement.
Journal ArticleDOI
Preparation of an Electron-dense Antibody Conjugate
TL;DR: This work has shown that it is necessary to confer sufficient electron density upon an antibody molecule, without inactivating it, to render it visible in the electron microscope.
Journal ArticleDOI
Membrane vesiculation as a feature of the mammalian acrosome reaction.
TL;DR: Data have now been obtained with glutaraldehyde-fixed tissue which show that extensive vesiculation takes place between plasma and acrosome membranes in the course of the acrosomes reaction evinced by hamster and rabbit spermatozoa as they pass through the egg investments.
Journal ArticleDOI
In vitro capacitation of hamster spermatozoa by follicular fluid
TL;DR: When hamster epididymal spermatozoa were incubated in vitro in the presence of follicular fluid in Tyrode's solution for 3 hr or more, they became fully capacitated and displayed an extremely vigorous motility.
Related Papers (5)
Electron microscope studies of sperm incorporation into the golden hamster egg.
Ryuzo Yanagimachi,Y. D. Noda +1 more