Electroporation of cell membranes.
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TLDR
Electroporation has found applications in introduction of plasmids or foreign DNA into living cells for gene transfections, fusion of cells to prepare heterokaryons, hybridoma, hybrid embryos, and constructing animal model by fusing human cells with animal tissues.About:
This article is published in Biophysical Journal.The article was published on 1991-08-01 and is currently open access. It has received 1053 citations till now. The article focuses on the topics: Electroporation & Electrocution.read more
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Applied Plasma Medicine
Gregory Fridman,Gary Friedman,Alexander Gutsol,Anatoly B. Shekhter,Victor N. Vasilets,Alexander Fridman +5 more
TL;DR: Non-equilibrium plasmas will be shown to be non-destructive to tissue, safe, and effective in inactivation of various parasites and foreign organisms.
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Theory of electroporation: A review
TL;DR: This review of the essential features of theoretical models of electroporation focuses on transient aqueous pore models, which can account for key features of mechanical instability and dramatic reversible electrical behavior of certain planar membranes and of cell membranes, and some features of molecular transport.
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Transdermal drug delivery: overcoming the skin's barrier function.
TL;DR: Overcoming this barrier function then, for the purpose of transdermal drug delivery, has been a necessarily challenging task for the pharmaceutical scientist, and one that boasts significant progress.
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Electroporation-Based Technologies for Medicine: Principles, Applications, and Challenges
TL;DR: This review describes the theory and current applications of electroporation in medicine and then discusses current challenges in Electroporation research and barriers to a more extensive spread of these clinical applications.
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Electroporation: A general phenomenon for manipulating cells and tissues
TL;DR: Electroporation of isolated cells has also been used for introduction of enzymes, antibodies, and other biochemical reagents for intracellular assays, and more recently, tissue electroporation has begun to be explored, with potential applications including enhanced cancer tumor chemotherapy, gene therapy, transdermal drug delivery, and noninvasive sampling for biochemical measurement.
References
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Gene transfer into mouse lyoma cells by electroporation in high electric fields.
TL;DR: A simple physical model for the enhanced DNA penetration into cells in high electric fields is proposed, according to which the interaction of the external electric field with the lipid dipoles of a pore configuration induces and stabilizes the permeation sites and thus enhances cross membrane transport.
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Specific intermediates in the folding reactions of small proteins and the mechanism of protein folding.
Peter S. Kim,Robert L. Baldwin +1 more
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Enhancer-dependent expression of human kappa immunoglobulin genes introduced into mouse pre-B lymphocytes by electroporation.
TL;DR: It is found that there is a B-cell-specific enhancer-activator region in the J-C intron of the human kappa gene that is necessary for efficient transcription of the cloned gene in mouse pre-B lymphocytes, suggesting that both the DNA element and the proteins required for its regulatory activity have been highly conserved in evolution.