Journal ArticleDOI
Estimation of protein secondary structure and error analysis from circular dichroism spectra.
Ivo H. M. van Stokkum,H.J.W. Spoelder,Michael Bloemendal,Rienk van Grondelle,Frans C. A. Groen +4 more
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TLDR
The adequacy of thelinear model is investigated, paying special attention to the estimation of the error in the secondary structure estimates, and it is shown that the linear model is only adequate for the alpha-helix class.About:
This article is published in Analytical Biochemistry.The article was published on 1990-11-15. It has received 476 citations till now. The article focuses on the topics: Linear model.read more
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Journal ArticleDOI
Estimation of Protein Secondary Structure from Circular Dichroism Spectra: Comparison of CONTIN, SELCON, and CDSSTR Methods with an Expanded Reference Set
TL;DR: Analyzing protein CD spectra using all three methods should improve the reliability of predicted secondary structural fractions, and three programs are provided in CDPro software package and have been modified for easier use with the different reference sets described in this paper.
Journal ArticleDOI
DICHROWEB, an online server for protein secondary structure analyses from circular dichroism spectroscopic data.
Lee Whitmore,Bonnie A. Wallace +1 more
TL;DR: The DICHROWEB web server enables on-line analyses of circular dichroism (CD) spectroscopic data, providing calculated secondary structure content and graphical analyses comparing calculated structures and experimental data.
Journal ArticleDOI
Protein secondary structure analyses from circular dichroism spectroscopy: Methods and reference databases
Lee Whitmore,Bonnie A. Wallace +1 more
TL;DR: Recent developments in the analysis of protein secondary structures, including features of the DICHROWEB analysis webserver are discussed, which greatly improve and facilitate the analyses of CD spectra.
Book ChapterDOI
[4] Circular dichroism
TL;DR: Circular dichroism is a spectroscopic method which depends on the fact that certain molecules interact differently with right and left circularly polarized light and can provide information about the secondary structure of proteins and nucleic acids and about the binding of ligands to these types of macromolecule.
Journal ArticleDOI
Obtaining information about protein secondary structures in aqueous solution using Fourier transform IR spectroscopy
TL;DR: The principles that underlie the determination of protein secondary structure by FTIR spectroscopy are detailed, as well as the basic steps involved in protein sample preparation, instrument operation,FTIR spectra collection and spectra analysis in order to estimate protein secondary-structural components in aqueous solution.
References
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Book
Solving least squares problems
TL;DR: Since the lm function provides a lot of features it is rather complicated so it is going to instead use the function lsfit as a model, which computes only the coefficient estimates and the residuals.
Journal ArticleDOI
Computed circular dichroism spectra for the evaluation of protein conformation
Journal ArticleDOI
Solving Least Squares Problems
Journal ArticleDOI
Estimation of globular protein secondary structure from circular dichroism.
S W Provencher,J Glöckner +1 more
TL;DR: In this article, a linear combination of the CD spectra (from 190 to 240 nm) of 16 proteins whose secondary structures are known from X-ray crystallography was used to characterize helix, beta sheet, beta turn, and remainder.
Related Papers (5)
Estimation of globular protein secondary structure from circular dichroism.
S W Provencher,J Glöckner +1 more
DICHROWEB, an online server for protein secondary structure analyses from circular dichroism spectroscopic data.
Lee Whitmore,Bonnie A. Wallace +1 more