Journal ArticleDOI
Evaluation of analytical techniques to predict viability after cryopreservation
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TLDR
Electrolyte leakage and TTC-staining are efficient to predict viability and a mathematical model allows us to save time and plant material in order to develop an efficient encapsulation—dehydration protocol.Abstract:
Preservation of plant germplasm is important to safeguard biodiversity and to store elite plants. Cryopreservation is one of the possible preservation techniques. Research for a cryopreservation protocol is often inefficient because of slow or poor regrowth of plant material. Therefore, at least one technique, that allows a quick and accurate prognosis of viability after cryopreservation, is required. We evaluated five techniques: electrolyte leakage, triphe-nyltetrazoliumchloride (TTC) staining (visual and spectrophotometrical analysis), malondialdehyde concentrations in plant tissue and a mathematical model that relates ‘water content’ to the weight of encapsulated plant material. Electrolyte leakage and TTC-staining (if visually analysed) are efficient to predict viability. Our mathematical model allows us to save time and plant material in order to develop an efficient encapsulation—dehydration protocol. All other techniques were rejected because of the high variability of the results. This is due to the variability of biochemical activity in plant tissue and the small amount of tissue used in the experiments.read more
Citations
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Journal ArticleDOI
Initiation, growth and cryopreservation of plant cell suspension cultures
TL;DR: A two-step (controlled rate) freezing technique also known as the slow (equilibrium) freezing method for long-term storage, which has been applied successfully to a wide range of plant cell suspension cultures is provided.
Journal ArticleDOI
Cryopreservation of protocorm-like bodies (PLBs) of Phalaenopsis bellina (Rchb.f.) christenson by encapsulation-dehydration
Amir Ali Khoddamzadeh,Uma Rani Sinniah,Paul T. Lynch,Midhzar Abdul Kadir,S. B. Kadzimin,Maziah Mahmood +5 more
TL;DR: Protocorm-like bodies (PLBs) of Phalaenopsis bellina were successfully cryopreserved by the encapsulation-dehydration approach and viability was determined by TTC reduction and re-growth assessed.
Journal ArticleDOI
Cryopreservation of Quercus suber somatic embryos by encapsulation-dehydration and evaluation of genetic stability
Pedro Fernandes,Eleazar Rodriguez,Glória Pinto,Isabel Roldán-Ruiz,Marc De Loose,Conceição Santos +5 more
TL;DR: An encapsulation and dehydration procedure for the cryopreservation of cork oak (Quercus suber L.) somatic embryos that resulted in at least 90% survival and potential for use in long-term conservation programs is described.
Journal ArticleDOI
Effects of osmotic pretreatments on oxidative stress, antioxidant profiles and cryopreservation of olive somatic embryos.
Paul T. Lynch,Ayesha Siddika,Jason W. Johnston,Susan M. Trigwell,Aradhana Mehra,Carla Benelli,Maurizio Lambardi,Erica E. Benson +7 more
TL;DR: These studies suggest oxidative processes may influence regrowth of cryopreserved olive SE and that optimal pretreatments could, in part, increase tolerance by an overall enhancement of endogenous antioxidants (particularly GR), proline and sugars.
Journal ArticleDOI
Cryopreservation of embryogenic tissues of Picea omorika (Serbian spruce)
TL;DR: Two-year-old embryogenic tissues of P. omorika (Pančić) Purk were successfully cryopreserved after preculture with sucrose, air-drying for 2 h, and freezing in liquid nitrogen (LN), and the frequency of bacterial and fungal contamination was higher if ET was frozen in LN-containing vials than in Ln-free vials.
References
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Journal ArticleDOI
Cryopreservation of alginate-coated in vitro-grown shoot tips of apple, pear and mulberry
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TL;DR: Alginate-coated shoot tips from in vitro-grown apple (Malus domestica Borkh cv. Fuji) were successfully cryopreserved following dehydration following encapsulation-dehydration, and this modified method appears to be a promising technique forCryopreserving shoot tip from in vivo-grown plantlets of deciduous trees.
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