Journal ArticleDOI
Fluorescence quenching studies with proteins.
TLDR
A review of the use of the technique of solute fluorescence quenching to study the structure and dynamics of proteins and a number of factors are discussed that must be considered in analyzing such data.About:
This article is published in Analytical Biochemistry.The article was published on 1981-07-01. It has received 1644 citations till now. The article focuses on the topics: Quenching (fluorescence).read more
Citations
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Journal ArticleDOI
Hydrogen exchange and structural dynamics of proteins and nucleic acids
TL;DR: Though the structures presented in crystallographic models of macromolecules appear to possess rock-like solidity, real proteins and nucleic acids are not particularly rigid.
Journal ArticleDOI
Biogenic synthesis of silver nanoparticles and their synergistic effect with antibiotics: a study against gram-positive and gram-negative bacteria
Amanulla Mohammed Fayaz,Kulandaivelu Balaji,M. Girilal,Ruchi Yadav,Pudupalayam Thangavelu Kalaichelvan,Ramasamy Venketesan +5 more
TL;DR: The result showed that the combination of antibiotics with AgNPs have better antimicrobial effects, and a mechanism was also proposed to explain this phenomenon.
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Tris(phenanthroline)ruthenium(II) enantiomer interactions with DNA: Mode and specificity of binding
TL;DR: The results of these studies indicated that both isomers bind to DNA by a single mode, but the two isomers differ, however, in their effect on the hydrodynamic properties of DNA as measured by viscosity and, therefore, probably differ in their individual binding modes.
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Metal-triggered structural transformations, aggregation, and fibrillation of human alpha-synuclein. A possible molecular NK between Parkinson's disease and heavy metal exposure.
TL;DR: It is indicated that low concentrations of some metals can directly induce alpha-synuclein fibril formation and the potential for ligand bridging by polyvalent metal ions is proposed to be an important factor in the metal-induced conformational changes of alpha- synuclein.
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Interaction of drugs with bovine and human serum albumin
TL;DR: Fluorescence emission spectra of serum albumin in the presence of MMI or PTU, recorded at the excitation wavelengths 280 and 295 nm, clearly show that the studied drugs act as quenchers.
References
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Journal ArticleDOI
Quenching of fluorescence by oxygen. A probe for structural fluctuations in macromolecules.
TL;DR: In this article, the fluorescence of various fluorophores by molecular oxygen has been studied in aqueous and nonaqueous solutions equilibrated with oxygen pressures up to 100 atm.
Journal ArticleDOI
Dynamics of folded proteins
TL;DR: The dynamics of a folded globular protein have been studied by solving the equations of motion for the atoms with an empirical potential energy function and suggest that the protein interior is fluid-like in that the local atom motions have a diffusional character.
Journal ArticleDOI
Solute perturbation of protein fluorescence. The quenching of the tryptophyl fluorescence of model compounds and of lysozyme by iodide ion.
TL;DR: The results of the model compound study provide evidence for a mechanism that follows the classical Stern-Volmer law (1919), predominantly involving collisional quenching, and illustrate the importance of local charge and solvent viscosity.
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Dynamics of ligand binding to myoglobin
TL;DR: The nonexponential rebinding observed at low temperatures and in solid samples implies that the innermost barrier has a spectrum of activation energies, similar to how myoglobin achieves specificity and order.
Related Papers (5)
Solute perturbation of protein fluorescence. The quenching of the tryptophyl fluorescence of model compounds and of lysozyme by iodide ion.
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