Journal ArticleDOI
Freeze-Drying of Oxyhemoglobin: Protection Against Oxidation in the Presence of EDTA Salts, Sulfonic Acid Buffers, and Pantothenic Acid Derivatives
A. Fauvel,P. Labrude,C. Vigneron +2 more
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TLDR
The multiplicity of compounds that inhibit the denaturation of hemoglobin during freeze-drying indicates that their mode of action is nonspecific, during both desiccation and storage.About:
This article is published in Journal of Pharmaceutical Sciences.The article was published on 1984-06-01. It has received 4 citations till now. The article focuses on the topics: Methemoglobin & Sulfonic acid.read more
Citations
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Journal ArticleDOI
Protective effect of sucrose on spray drying of oxyhemoglobin.
TL;DR: Investigation by optical circular dichroism confirmed the results obtained by freeze drying: in the presence of an effective protector, the spectra were similar to those of control hemoglobin and the immediate environment of the heme did not undergo any major change.
Journal ArticleDOI
Formulation concerns of protein drugs
TL;DR: In this article, the authors discuss the formulation concerns of protein drugs and their application in the field of drug development and industrial pharmacology, and present a survey of the literature on protein drugs.
Journal ArticleDOI
Quality control material containing hemoglobin for blood gas and pH measurement: preparation of stroma-free hemoglobin solution.
TL;DR: During storage at 4 degrees C no methemoglobin was formed in contrast with two other types of stroma-free hemoglobin solution, which formed appreciable amounts of meethemoglobin within 40 days.
Preparation of dried hemoglobin without loss of activity.
TL;DR: The technique for freezing, drying, and preserving in vacuo which is in common use for plasma can be successfully applied to hemoglobin solutions when the hemoglobin is first deoxygenated to the extent of 99.7 per cent or more.
References
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Journal ArticleDOI
Microdetermination of oxyhemoglobin, methemoglobin, and sulfhemoglobin in a single sample of blood
TL;DR: The concentration of SHb in a solution containing HbOt, MHb, and SHb is proportional to the residual optical density of the solution after the MHb has been converted into MHbCN by addition of cyanide.
Journal ArticleDOI
Mechanism of protein stabilization by glycerol: preferential hydration in glycerol-water mixtures
TL;DR: A densimetric investigation of the interactions between solvent components in glycerol-water mixtures and seven proteins and all the proteins were found to be preferentially hydrated at all conditions used, i.e., addition of the proteins to the mixed solvent results in an increase in the chemical potential of Glycerol.
Journal ArticleDOI
Preparation of dried hemoglobin without loss of activity
TL;DR: The technique for freezing, drying, and preserving in vacuo which is in common use for plasma can be successfully applied to hemoglobin solutions when the hemoglobin is first deoxygenated to the extent of 99.7 per cent or more.
Journal ArticleDOI
Freeze-drying of haemoglobin in the presence of carbohydrates.
TL;DR: Salivary rather than serum bioavailability studies may be clinically more relevant, as cornparisons of free pharmacologically active drug fractions would appear to give a better Surement of a drug’s concentration a t its receptor site and better quantitation of clinical response.
Journal ArticleDOI
Circular dichroism studies of freeze-drying-induced conformational changes in human hemoglobin
TL;DR: Experimental data suggest that the protective effect of hemoglobin in the absence of a protector weakly influences the conformation in the vicinity of the heme and increases the helicity of protein chains from 75 to 81%.
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