Functional properties of the ryanodine receptor type 3 (RyR3) Ca2+ release channel.
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TLDR
The functional properties of the RyR3 channels are in agreement with a potential qualitative contribution of this channel to Ca2+ release in skeletal muscle and in other tissues.Abstract:
Single-channel analysis of sarcoplasmic reticulum vesicles prepared from diaphragm muscle, which contains both RyR1 and RyR3 isoforms, revealed the presence of two functionally distinct ryanodine receptor calcium release channels. In addition to channels with properties typical of RyR1 channels, a second population of ryanodine-sensitive channels with properties distinct from those of RyR1 channels was observed. The novel channels displayed close-to-zero open-probability at nanomolar Ca2+ concentrations in the presence of 1 mM ATP, but were shifted to the open conformation by increasing Ca2+ to micromolar levels and were not inhibited at higher Ca2+ concentrations. These novel channels were sensitive to the stimulatory effects of cyclic adenosine 5'-diphosphoribose (cADPR). Detection of this second population of RyR channels in lipid bilayers was always associated with the presence of the RyR3 isoform in muscle preparations used for single-channel measurements and was abrogated by the knockout of the RyR3 gene in mice. Based on the above, we associated the novel population of channels with the RyR3 isoform of Ca2+ release channels. The functional properties of the RyR3 channels are in agreement with a potential qualitative contribution of this channel to Ca2+ release in skeletal muscle and in other tissues.read more
Citations
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A novel cycling assay for cellular cADP-ribose with nanomolar sensitivity.
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Deletion of the ryanodine receptor type 3 (RyR3) impairs forms of synaptic plasticity and spatial learning
Detlef Balschun,David P. Wolfer,Federica Bertocchini,Virginia Barone,Antonio Conti,Werner Zuschratter,Ludwig Missiaen,Hans-Peter Lipp,J. Uwe Frey,Vincenzo Sorrentino +9 more
TL;DR: The observed physiological and behavioral effects implicate RyR3‐mediated Ca2+ release in the intracellular processes underlying spatial learning and hippocampal synaptic plasticity.
References
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Inositol trisphosphate and calcium signalling
TL;DR: Inositol trisphosphate is a second messenger that controls many cellular processes by generating internal calcium signals through receptors whose molecular and physiological properties closely resemble the calcium-mobilizing ryanodine receptors of muscle.
Journal ArticleDOI
Ryanodine Receptor/Ca2+ Release Channels and Their Regulation by Endogenous Effectors
TL;DR: Foot structures have been termed feet and are now commonly known as ryanodine receptor/Ca2+ release channels because of the presence of an intrinsic ci+ channel activity within the feet structures, and their ability to bind the plant.
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Stabilization of calcium release channel (ryanodine receptor) function by FK506-binding protein.
Anne-Marie B. Brillantes,Karol Ondrias,Karol Ondrias,Andrew Scott,Andrew Scott,Evgeny Kobrinsky,Evgeny Kobrinsky,Elena Ondriasova,Maria C. Moschella,Maria C. Moschella,Thottala Jayaraman,Thottala Jayaraman,Mark Landers,Mark Landers,Barbara E. Ehrlich,Barbara E. Ehrlich,Andrew R. Marks,Andrew R. Marks +17 more
TL;DR: These results provide the first natural cellular function for FKBP12, and establish that the functional Ca2+ release channel complex includes FK BP12.
Journal ArticleDOI
Ca(2+)-induced Ca2+ release in sea urchin egg homogenates: modulation by cyclic ADP-ribose.
TL;DR: Cyclic ADP-ribose, a metabolite with potent Ca(2+)-releasing properties, appears to act by way of the CICR mechanism and may thus be an endogenous modulator of C ICR.
Journal ArticleDOI
Structure and function of ryanodine receptors
TL;DR: The diversity of ligands known to modulate gating and the diversity of tissues known to express the protein suggest that the ryanodine receptor has the potential to participate in many types of cell stimulus-Ca(2+)-release coupling mechanisms.
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