Showing papers in "Journal of Biological Chemistry in 1987"

TL;DR: Small amounts of myoglobin, beta-lactoglobulin, and other proteins and peptides can be spotted or electroblotted onto polyvinylidene difluoride membranes, stained with Coomassie Blue, and sequenced directly, suggesting that PVDF membranes are superior supports for sequence analysis of picomole quantities of proteins purified by gel electrophoresis.

TL;DR: Genistein inhibited the EGF-stimulated increase in phosphotyrosine level in A431 cells and scarcely inhibited the enzyme activities of serine- and threonine-specific protein kinases such as cAMP-dependent protein kinase, phosphorylase kinases, and the Ca2+/phospholipid-dependent enzymeprotein kinase C.

TL;DR: The lipid composition of the vesicles shows the high sphingomyelin content characteristic of sheep red cell plasma membranes, but not white cell or platelet membranes, consistent with the conclusion that the vESicles are of reticulocyte origin.

TL;DR: The results indicate a general sensitivity of proteins to oxygen radicals and indicate that proteins which have been denatured by .OH can be recognized and degraded rapidly and selectively by intracellular proteolytic systems.

TL;DR: These studies indicate that both aspartic acid and asparagine residues may be hot spots for the nonenzymatic degradation of proteins, especially in cells such as erythrocytes and eye lens, where these macromolecules must function for periods of about 120 days and 80 years, respectively.

TL;DR: There is evidence that foreign DNA can be specifically delivered to cells by a soluble carrier system that takes advantage of receptor-mediated endocytosis, and that competition by a 10-fold excess of ASOR prevented gene transformation by the ASOR X poly-L-lysine X DNA complex.

TL;DR: The results suggest that loss of functional enzyme activity and increased heat lability of enzymes during aging may be due in part to oxidative modification by mixed function oxidation systems.

TL;DR: The findings indicate that the toxin inactivates the ribosomes by modifying both or either of two nucleoside residues, G4323 and A4324, in 28 S rRNA, in a general mechanistic pathway for ribosome inactivation by lectin toxins.

TL;DR: The results in the present experiments demonstrated that ricin A-chain inactivates the ribosomes by cleaving the N-glycosidic bond of A4324 of 28 S rRNA in a hydrolytic fashion.

TL;DR: The alterations to primary structure observed provide the key to an understanding of the link between oxidative modification and increased proteolytic susceptibility.

TL;DR: Carbohydrate binding properties of a new plant lectin isolated from elderberry bark were studied using the techniques of quantitative precipitation, hapten inhibition, and equilibrium dialysis, suggesting the presence of a hydrophibic interacting region adjacent to the carbohydrate binding site.

TL;DR: These studies lend support to the hypothesis that different Arg-Gly-Asp-directed adhesion receptors can recognize differences in the conformation and environment of the Arg- Gly- asp tripeptide, and they establish the feasibility of obtaining synthetic probes that are more selective for individual receptors than are the peptides modeled after the natural sequences of adhesive extracellular matrix molecules.

TL;DR: The results indicate that TGF-beta 1 controls the composition and abundance of extracellular matrices at least in part by inducing a coordinate increase in the levels of fibronectin and type I collagen mRNAs.

TL;DR: The expression of apolipoprotein E,E(LDL) receptors by brain cells and the presence of apoE- and apoA-I-containing lipoproteins in CSF suggest that the central nervous system has a mechanism for lipid transport and cholesterol homeostasis similar to that of other tissues.

TL;DR: In olfactory tissue, G alpha s was the most abundant of these messages and in combination with the biochemical studies suggests that Galpha s is the G-protein component of the olfaction signal transduction cascade.

TL;DR: Binding of the molecules to bovine serum albumin decreased the quenching constant for oxygen by approximately an order of magnitude and inhibited probe self-quenching, indicating that at the protein binding site the probes are somewhat protected from collision with quenchers.

TL;DR: Results support that TGF beta is an important and multifunctional autocrine regulator of bone formation.

TL;DR: It is suggested that oxidation of LDL is accompanied by derivatization of lysine epsilon-amino groups by lipid products and that these adducts may be important in the interaction of oxidized LDL with the acetyl-LDL receptor.

TL;DR: In this paper, the effects of substitution of alanine for lysine at amino acid 1018 in the ATP-binding domain of the beta subunit of the human β-subunit were investigated.

TL;DR: To establish the structural and functional properties of this receptor on M21 human melanoma cells, stable variant cell lines were selected that express altered alpha chain levels and a monoclonal antibody, LM609, is capable of preventing M21 cell attachment to vitronectin, von Willebrand factor, fibrinogen, and the Arg-Gly-Asp peptide but not to fibronECTin.

TL;DR: The present finding suggests that the ryanodine receptor and Ca2+ release channel represent a functional unit, the structural unit being the foot structure which, in situ, is junctionally associated with the transverse tubules and across this triad junction that the signal for Ca1+ release is expressed.

TL;DR: Combinations of various chemical radical scavengers revealed that .OH is the primary species responsible for alteration of BSA secondary and tertiary structure and may hold important clues for the mechanism(s) by which oxygen radicals can increase proteolytic susceptibility.

TL;DR: It is found that a soluble egg factor converts NAD into a highly active metabolite that releases Ca2+ without a lag, and this metabolite was purified to homogeneity by high pressure liquid chromatography and produced half-maximal Ca2 + release at about 40 nM.

TL;DR: Using Fe2+, H2O2, and phospholipid liposomes as a model system, it is found that lipid peroxidation, as assessed by malondialdehyde formation, is not initiated by the hydroxyl radical, but rather requires Fe3+ and Fe2+.

TL;DR: In this article, two high molecular weight proteases were obtained from rabbit reticulocyte lysate, one having a molecular weight of 1,000,000 +/- 100,000 and the other having a lower molecular weight.

TL;DR: The calcium channel blockers verapamil and diltiazem inhibited the vinblastine photoaffinity labeling of P170, the protein previously shown to be a marker of multidrug resistance.

TL;DR: Evidence reported here strongly suggests that in vitro inhibition of phospholipase A2 by lipocortin is due to sequestering of theospholipid substrate by the inhibitor protein, rather than a direct interaction with the phospholIPase.

TL;DR: It is shown that the carbohydrate moiety of urinary erythropoietin is indistinguishable from recombinant eryanthropoietIn except for a slight difference in sialylation, providing the evidence that recombinant ______ is valuable for biological as well as clinical use.

TL;DR: Results suggest that actions of inositol 1,4,5-trisphosphate are regulated by physiological alterations in intracellular pH and calcium concentrations.

TL;DR: Evidence for five distinct VLA alpha subunits was obtained from differences observed in antibody recognition, cell distribution patterns, two-dimensional gel analyses, and V8 protease cleavage patterns.