Gas-Liquid Chromatographic Isolation of Cytokinins from Natural Sources 6-(3-Methyl-2-butenylamino) purine from Agrobacterium Tumefaciens
TLDR
Ethyl acetate extraction of yeast transfer RNA hydrolysates and of culture filtrates of Agrobacterium tumefaciens gave sufficient concentration of the naturally occurring cytokinins for immediate gas-liquid chromatography, and an active cytokinin was isolated and was tentatively identified as 6-(3-methyl-2-butenylamino)purine.Abstract:
Gas-liquid chromatographic retention times for 20 purines or purine nucleosides, 14 of which are highly active cytokinins, are reported. With one exception, all of the naturally occurring cytokinins are separated.Ethyl acetate extraction of yeast transfer RNA hydrolysates and of culture filtrates of Agrobacterium tumefaciens gave sufficient concentration of the naturally occurring cytokinins for immediate gas-liquid chromatography. This procedure permitted the detection of 6-(3-methyl-2-butenylamino)-9-beta, d-ribofuranosylpurine in yeast transfer RNA extracts. An active cytokinin was isolated from A. tumefaciens culture filtrates and was tentatively identified as 6-(3-methyl-2-butenylamino)purine.read more
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Identification of plant hormones from cotton ovules.
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TL;DR: An extract from 8-day-old cotton ovules was partitioned into three fractions and each fraction was derivatized and analyzed separately to separate, measure, and identify the naturally occurring plant hormones.
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Algae and cytokinins
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Products of Soil Microorganisms in Relation to Plant Growth
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Cytokinin production by microorganisms
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