Journal ArticleDOI
Genetic variability between different accessions of some common commercial olive cultivars
TLDR
Leaves of 22 accessions of olive from three named cultivars, and 17 accessions from cultivars with names that are suspected to include synonyms, showed a within-cultivar genetic similarity of at least 98% irrespective of source, while Verdale showed a high genetic variability, with some accessions having less than 80% similarity to others.Abstract:
SummaryLeaves of 22 accessions of olive (Olea europaea L.) from three named cultivars (Manzanillo, Kalamata, and Verdale), and 17 accessions from cultivars with names that are suspected to include synonyms (Corregiola, Corregiolo, Frantoio, Frantoja, Frantojol, Nevadillo, Nevadillo Blanco, and Picual) were obtained from Israel, Italy, the USA and Australia. Their genetic variability was investigated using random amplified polymorphic DNA (RAPD). Analysis of the PCR products was achieved by using the simple matching coefficient and UPGMA clustering. `Manzanillo' and `Kalamata' showed a within-cultivar genetic similarity of at least 98% irrespective of source. In contrast, `Verdale' showed a high genetic variability, with some accessions having less than 80% similarity to others. `Picual' and `Nevadillo' (including `Nevadillo Blanco'), which clustered at 69%, are possibly distinct cultivars. The identity of `Corregiola' and `Frantoio' and their synonyms is uncertain. Considering the extent of the genetic di...read more
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Comparative study of the discriminating capacity of RAPD, AFLP and SSR markers and of their effectiveness in establishing genetic relationships in olive
Angjelina Belaj,Zlatko Šatović,Guido Cipriani,Luciana Baldoni,Raffaele Testolin,Luis Rallo,Isabel Trujillo +6 more
TL;DR: SSRs presented a higher level of polymorphism and a greater information content, as assessed by the expected heterozygosity, than AFLPs and RAPDs, and were the most efficient marker system in a study of genetic diversity and relationships among 32 olive cultivars cultivated in Italy and Spain.
Journal ArticleDOI
Polymorphism and Discrimination Capacity of Randomly Amplified Polymorphic Markers in an Olive Germplasm Bank
TL;DR: Three major groups of cultivars could be distinguished by RAPD analysis: 1) cultivars from east and northeast Spain, 2) Turkish, Syrian, and Tunisian cultivars, and 3) the majority of common olive cultivars in Spain.
Journal ArticleDOI
Cultivar identification and genetic fingerprinting of temperate fruit tree species using DNA markers
Ana Wünsch,José I. Hormaza +1 more
TL;DR: The information so far published in molecular fingerprinting of temperate fruit tree species using DNA markers is analysed with the goal of obtaining a common ground that will allow an easier and faster genetic identification that, at the same time, has to be reproducible among laboratories.
Journal ArticleDOI
High resolution melting analysis of almond SNPs derived from ESTs
TL;DR: High resolution melting curve analysis was applied to almond SNP discovery and genotyping based on the predicted SNP information derived from the almond and peach EST database to demonstrate its ability to detect variation as small as one base difference between samples.
Journal ArticleDOI
Genetic diversity and relationships in olive ( Olea europaea L.) germplasm collections as determined by randomly amplified polymorphic DNA.
TL;DR: The importance of the study of the amount and distribution of genetic diversity for a better exploration of olive genetic resources and the design of plant breeding programmes is indicated.
References
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Journal ArticleDOI
Fast and sensitive silver staining of DNA in polyacrylamide gels
TL;DR: The photochemically derived silver stain of nucleic acids in polyacrylamide gels originally described by Merril et al. was modified to reduce unspecific background staining and increase sensitivity and this improved staining procedure is applied for the routine analysis of complex DNA profiles generated by DNA amplification fingerprinting (DAF).