Journal ArticleDOI
Genetic variation at allozyme and RAPD loci in sessile oak Quercus petraea (Matt.) Liebl.: the role of history and geography
TLDR
Although RAPDs revealed less genetic diversity than allozymes, levels of genetic differentiation (GST) were identical and possibly, divergent selection at two allozyme loci during the glacial period could explain this pattern.Abstract:
The nuclear genetic variation within and among 21 populations of sessile oak was estimated at 31 RAPD loci in conjunction with previous estimates of variation at eight allozyme loci. The aim of the study was to assess the relative role of isolation-by-distance and postglacial history on patterns of nuclear variation. Because of its small effective population size and maternal transmission, the chloroplast genome is a good marker of population history. Both kinds of nuclear variation (RAPD and allozyme) were therefore compared, first, to the geographical distances among populations and, secondly, to chloroplast DNA restriction polymorphism in the same populations. Multiple Mantel tests were used for this purpose. Although RAPDs revealed less genetic diversity than allozymes, levels of genetic differentiation (GST) were identical. The standard genetic distance calculated at all RAPD loci was correlated with geographical distances but not with the genetic distance calculated from chloroplast DNA data. Conversely, allozyme variation was correlated with chloroplast DNA variation, but not with geography. Possibly, divergent selection at two allozyme loci during the glacial period could explain this pattern. Because of its greater number of loci assayed, RAPDs probably provided a less biased picture of the relative role of geography and history.read more
Citations
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Journal ArticleDOI
The effect of Quaternary climatic changes on plant distribution and evolution
TL;DR: A comparative analysis shows that phylogeographic patterns in Europe appear to be less congruent than in North America, and requires further research into the estimation of reliable and accurate divergence times.
Journal ArticleDOI
Effects of life history traits and sampling strategies on genetic diversity estimates obtained with RAPD markers in plants
Hilde Nybom,Igor V. Bartish +1 more
TL;DR: The results suggest that RAPD can be a sensitive method for detection of genetic structuring according to the isolation-by-distance model, but also means that sampling strategies, as applied in individual studies, can seriously influence the resulting estimates of between-population diversity.
Book
DNA fingerprinting in plants: principles, methods, and applications
TL;DR: Amin et al. as discussed by the authors used radioisotopes to measure the diversity of microsatellites in the field of plant DNA collection and preservation of plant Tissue in the Field.
Journal ArticleDOI
Effects of Colonization Processes on Genetic Diversity: Differences Between Annual Plants and Tree Species
Frédéric Austerlitz,Stéphanie Mariette,Nathalie Machon,Pierre-Henri Gouyon,Bernard Godelle,Bernard Godelle +5 more
TL;DR: It is shown that both a reasonably high level of pollen flow and the life-cycle characteristics of trees are needed to explain the observed structure of genetic diversity and that gene flow and life cycle both have an impact on maternally inherited cytoplasmic genes.
Journal ArticleDOI
The distribution of random amplified polymorphic DNA (RAPD) diversity amongst populations of Isotoma petraea (Lobeliaceae)
TL;DR: Non‐metric multidimensional scaling (nMDS) analysis of the random amplified polymorphic DNA (RAPD) data set clearly resolved all populations and found there was little diversity in highly autogamous populations, but levels were higher in the outbred Yackeyackine population.
References
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Journal ArticleDOI
The neighbor-joining method: a new method for reconstructing phylogenetic trees.
Naruya Saitou,Masatoshi Nei +1 more
TL;DR: The neighbor-joining method and Sattath and Tversky's method are shown to be generally better than the other methods for reconstructing phylogenetic trees from evolutionary distance data.
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Molecular Evolutionary Genetics
TL;DR: Recent developments of statistical methods in molecular phylogenetics are reviewed and it is shown that the mathematical foundations of these methods are not well established, but computer simulations and empirical data indicate that currently used methods produce reasonably good phylogenetic trees when a sufficiently large number of nucleotides or amino acids are used.
Journal ArticleDOI
DNA polymorphisms amplified by arbitrary primers are useful as genetic markers
TL;DR: A new DNA polymorphism assay based on the amplification of random DNA segments with single primers of arbitrary nucleotide sequence is described, suggesting that these polymorphisms be called RAPD markers, after Random Amplified Polymorphic DNA.
Journal Article
The Detection of Disease Clustering and a Generalized Regression Approach
TL;DR: The technic to be given below for imparting statistical validity to the procedures already in vogue can be viewed as a generalized form of regression with possible useful application to problems arising in quite different contexts.
Journal ArticleDOI
Analysis of Gene Diversity in Subdivided Populations
TL;DR: A method is presented by which the gene diversity (heterozygosity) of a subdivided population can be analyzed into its components, i.e., the gene diversities within and between subpopulations.