Journal ArticleDOI
Genomic damage as an independent predictor marker of mortality in hemodialysis patients.
Elisabet Coll,Elitsa Stoyanova,Lara Rodríguez-Ribera,Miriam Solozábal,Susana Pastor,Irene Silva,Juan Manuel Diaz,José Ballarín,Noel Xamena,Ricard Marcos +9 more
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TLDR
It is shown for the first time that the presence of high levels of genomic damage is a strong predictor of all-cause mortality in HD patients and this association remains significant after adjustment for relevant covariates.Abstract:
AIM Hemodialysis (HD) patients present an enhanced mortality. Since oxidative DNA damage can be considered a biomarker of genomic instability our aim was to evaluate the influence of this genetic biomarker in all-cause mortality in a group of HD patients followed for 4 years. MATERIAL AND METHODS 123 chronic HD patients were included. Overall genomic damage was analyzed using the Comet assay. Oxidative DNA damage was measured using the Comet assay complemented with the use of Endo-III and FPG enzymes. Follow-up was carried out from January 2007 to July 2011. RESULTS Selected HD patients had a mean age of 62 ± 15 years. During the follow-up 36% of patients died (48% due to cardiovascular disease) and 23% were transplanted. Older patients, with high CRP levels, low levels of cholesterol-HDL and albumin, and higher genetic damage at the beginning of the study showed an increased risk for mortality. Multivariate analysis showed that only genomic damage, age and CRP were independently associated with mortality. CONCLUSIONS Our study shows for the first time that, in HD patients, the presence of high levels of genomic damage is a strong predictor of all-cause mortality. This association remains significant after adjustment for relevant covariates.read more
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Journal ArticleDOI
Measurement of DNA damage with the comet assay in high-prevalence diseases: current status and future directions.
TL;DR: This review assesses the association between high-prevalence diseases in high-income countries and DNA damage measured with the comet assay in humans and finds that patients with coronary artery disease, diabetes, kidney disease and chronic obstructive pulmonary disease have on average 2- to 3-fold higher levels of oxidatively damaged DNA in white blood cells than controls.
Journal ArticleDOI
DNA damage in non-communicable diseases: A clinical and epidemiological perspective
Mirta Milić,Alessandra Frustaci,Alessandra Del Bufalo,Juana Sánchez-Alarcón,Rafael Valencia-Quintana,Patrizia Russo,Stefano Bonassi +6 more
TL;DR: The present review will gather some of the most relevant knowledge concerning the presence of DNA damage in NCDs focusing on cardiovascular diseases, diabetes, chronic obstructive pulmonary disease, and neurodegenerative disorders, and discussing a selection of papers from the most informative literature.
Journal ArticleDOI
Genomic damage as a biomarker of chronic kidney disease status
Zuray Corredor,Elitsa Stoyanova,Lara Rodríguez-Ribera,Elisabet Coll,Irene Silva,Juan Manuel Diaz,José Ballarín,Ricard Marcos,Susana Pastor +8 more
TL;DR: It is confirmed that genomic damage can be predictive of prognosis in CKD patients, with high levels of DNA damage indicating a poor prognosisIn HD patients, although HD patients showed significantly higher levels of oxidative damage than pre‐HD patients.
Journal ArticleDOI
Association of elevated frequency of micronuclei in peripheral blood lymphocytes of type 2 diabetes patients with nephropathy complications.
TL;DR: The results indicate that increased genomic instability expressed as MNs is associated with nephropathy in all pathological stages and implementation of MN assay in clinical level may potentially enhance the quality of management of patients with diabetes and its complications such as nephtropathy.
Journal ArticleDOI
Vitamin E-coated dialysis membranes reduce the levels of oxidative genetic damage in hemodialysis patients.
Lara Rodríguez-Ribera,Zuray Corredor,Irene Silva,Juan Manuel Diaz,José Ballarín,Ricard Marcos,Susana Pastor,Elisabet Coll +7 more
TL;DR: The use of vitamin E-coated membranes supposes a decrease on the levels of oxidative DNA damage, and improves the uremic anemia status.