Journal ArticleDOI
Glycoprotein synthesis in the subcommissural organ of the chick embryo. I. An ontogenetical study using specific antibodies.
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In this article, the specificity of the antibodies was controled by immunostaining and by a competition test between lectins (Concanavalin A-Con A- and wheat germ agglutinin-WGA-) and antibodies (A74 IgG).Abstract:
Antibodies were raised in rabbit against crude subcommissural organ (SCO) extract of 19 day old chick embryos. After absorption with crude brain extract, the IgG fraction was purified by ion exchange chromatography. The specificity of the antibodies was controled by immunostaining and by a competition test between lectins (Concanavalin A-Con A- and wheat germ agglutinin-WGA-) and antibodies (A74 IgG).read more
Citations
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Soluble material from Reissner's fiber displays anti-aggregative activity in primary cultures of chick cortical neurons
TL;DR: Modulation of cell-cell interactions by SCO/RF glycoproteins strengthens the hypothesis of the involvement of RF in developmental events of the central nervous system.
Journal ArticleDOI
Ontogenesis of the secretory epithelium of the bovine subcommissural organ. A histofluorescence study using lectins and monoclonal antibodies
TL;DR: The secretory ependymal cells in the SCO express a particular phenotype and could represent an increasing model to study cell differentiation in the brain, and molecular characterization of subcommissuralin and experimental analyses on its accurate role in brain development will further the tentative comparison with ependedymins.
Journal ArticleDOI
In vitro differentiation of chick spinal cord neurons in the presence of Reissner's fibre, an ependymal brain secretion
TL;DR: The results suggest a direct modulation of cell-cell interactions by SCO/RF glycoproteins and an indirect survival effect on neurons, which strengthen the hypothesis of the involvement of SCo/RF complex in the development of the central nervous system (CNS).
Journal ArticleDOI
Floor plate and the subcommissural organ are the source of secretory compounds of related nature: comparative immunocytochemical study.
Carlos R. Yulis,Mota,J.A. Andrades,Sara Rodríguez,Bruno Peruzzo,Juan Miguel Mancera,P. Ramirez,M. Garrido,J.M. Pérez‐Fígarez,Pedro Fernández-Llebrez,E. M. Rodríguez +10 more
TL;DR: Evidence was obtained that the FP secretes into the cerebrospinal fluid a material chemically related to the RF‐glycoproteins secreted by the subcommissural organ, suggesting that in addition to being the source of contact‐mediated and diffusible signals, the FP might also secrete compounds into the cerebral fluid that may act on distant targets.
Journal ArticleDOI
Glycoprotein synthesis in the subcommissural organ of the chick embryo. II. An immunochemical study.
TL;DR: In the chick embryo, A74 immunoaffinity chromatography allowed to purify specific glycoproteins relevant to the SCO ventricular secretory process to discuss the known biosynthesis pathway of complex type glycoproteinins.
References
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Journal ArticleDOI
A series of normal stages in the development of the chick embryo
TL;DR: The preparation of a series of normal stages of the chick embryo does not need justification at a time when chick ernbryos are not only widely used in descriptive and experimental embryology but are proving to be increasingly valuable in medical research, as in work on viruses and cancer.
Journal ArticleDOI
A series of normal stages in the development of the chick embryo
TL;DR: In this article, a series of normal stages of the chick embryo is described in terms of the length of time of incubation, except for the first three days during which more detailed characteristics such as the number of somites are applied.
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The subcommissural organ.
TL;DR: The subcommissural organ (SCO) is a phylogenetically ancient and conserved structure that is one of the first brain structures to differentiate and may participate in different processes such as the clearance of certain compounds from theCSF, the circulation of CSF, and morphogenetic mechanisms.