Journal ArticleDOI
Growth regulator effects on bud initiation in callus cultures of medicago sativa
J. W. Saunders,E. T. Bingham +1 more
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There were several indications that many of the buds were initiated on the first medium and completed development on the second medium, including the differential effect on budding of combinations of 2,4-D, NAA and kinetin in the callus initiation medium, the specific media sequence required, and the presence of embryoids on thecallus which after transfer to the yeast extract-inositol medium produced buds.Abstract:
concentrations (0-36AM) of kinetin (K), six concentrations (0-44,UM) of naphthaleneacetic acid (NAA), and seven concentrations (0-36,UM) of 2,4-dichlorophenoxyacetic acid (2,4-D). After 28 days the callus was challenged to form buds by transfer to the modified Blaydes' medium containing 2.0 g/liter yeast extract and 0.57 mm inositol. No buds were produced in the absence of 2,4-D in the first medium, and the frequency of bud formation on the second medium was directly proportional to the 2,4-D concentration in the range 2.3-54 uM in the preceding medium. Buds were produced in the absence of kinetin in the first medium, but its presence in the range 2.3-36,uM markedly increased bud formation. NAA was not required for bud formation, and the budding frequency increased only slightly with increasing NAA concentration in the first medium. Budding of callus of two other alfalfa clones was also influenced by the 2,4-D concentration in the initial medium. There were several indications that many of the buds were initiated on the first medium and completed development on the second medium. These included the differential effect on budding of combinations of 2,4-D, NAA and kinetin in the callus initiation medium, the specific media sequence required, and the presence of embryoids on the callus which after transfer to the yeast extract-inositol medium produced buds.read more
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Book ChapterDOI
Morphogenic Aspects of Somatic Embryogenesis
TL;DR: Credit for the first descriptions of somatic embryogenesis might more properly belong to Levine, who in 1947 reported the recovery of carrot “seedlings” from tissues exposed to low levels of α-naphthaleneacetic acid, via a process whose description sounds very much like somatic embryos.
Book ChapterDOI
Plant Growth Regulators II: Cytokinins, their Analogues and Antagonists
TL;DR: Hormones in plants differ from most of those in animals by having pleiotropic effects; that is, they are involved in the control of a wide range of developmental processes, but the effect of a hormone on any developmental process depends on the species.
Journal ArticleDOI
Plant Regeneration from Mesophyll Protoplasts of Alfalfa
K.N. Kao,M.R. Michayluk +1 more
TL;DR: Embryoids were initiated from alfalfa mesophyll protoplasts and subsequently regenerated to plants and the ability of these protoplast to form embryoids and regenerate into plants varied considerably from plant to plant within the same cultivar.
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Genotype Specificity of Somatic Embryogenesis and Regeneration in Maize
TL;DR: It is demonstrated that nuclear genes exhibiting dominance are important in the formation of somatic embryos and in regeneration of corn plants.
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Organogenesis and somatic embryogenesis in tissues derived from leaf protoplasts and leaf expiants of Medicago sativa
TL;DR: In this paper, the developmental pattern was similar to that of callus initiated from leaves of axenic regenerated plants also retained its totipotency, in relation to genetic manipulations in forage legumes.
References
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Journal ArticleDOI
A revised medium for rapid growth and bio assays with tobacco tissue cultures
Toshio Murashige,Folke Skoog +1 more
TL;DR: In vivo redox biosensing resolves the spatiotemporal dynamics of compartmental responses to local ROS generation and provide a basis for understanding how compartment-specific redox dynamics may operate in retrograde signaling and stress 67 acclimation in plants.
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Organic Growth Factor Requirements of Tobacco Tissue Cultures
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Streptomycin-resistant Plants from Callus Culture of Haploid Tobacco
TL;DR: Conditions are worked out for the isolation of streptomycin-resistant tobacco cell lines, from which diploid fertile plants carrying the streptomecin- resistant character, were regenerated and seedlings grown from seeds of self-fertilized resistant Str-r1 plants were resistant.
Journal ArticleDOI
Parasexual Interspecific Plant Hybridization
TL;DR: The biochemical and morphological characteristics of the somatically produced hybrid were identical to those of the sexually produced amphiploid.
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Induction of Haploid Rice Plant from Anther Culture
Hiroo Niizeki,Kiyoharu Oono +1 more
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