Streptomycin-resistant Plants from Callus Culture of Haploid Tobacco

TLDR: Conditions are worked out for the isolation of streptomycin-resistant tobacco cell lines, from which diploid fertile plants carrying the streptomecin- resistant character, were regenerated and seedlings grown from seeds of self-fertilized resistant Str-r1 plants were resistant.

Abstract: THERE are few reports of the isolation of biochemical mutants from cultured plant cells1–3. We know of none describing plants which have been regenerated from these mutant cell lines and which still carry the mutant trait. We have worked out conditions for the isolation of streptomycin-resistant tobacco cell lines, from which diploid fertile plants carrying the streptomycin-resistant character, were regenerated. Seedlings grown from seeds of self-fertilized resistant Str-r1 plants were also resistant. The progeny obtained from crosses of Str-r1 plants and streptomycin sensitive (Str-s) plants showed non-Mendelian, uniparental transmission of the resistance trait.