Journal ArticleDOI
Haemangiopericytomas: the prognostic value of immunohistochemical staining with a monoclonal antibody to proliferating cell nuclear antigen (PCNA).
C. C.-W. Yu,P. A. Hall,P. A. Hall,Christopher D.M. Fletcher,Christopher D.M. Fletcher,R. S. Camplejohn,R. S. Camplejohn,N. H. Waseem,N. H. Waseem,David P. Lane,David P. Lane,David A. Levison +11 more
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TLDR
Staining with PC10 may be of particular value in the identification of patients at greatest risk of rapid tumour metastasis and early death in cases of haemangiopericytoma.Abstract:
Forty-two cases of haemangiopericytoma were studied retrospectively using immunohistochemical staining with PC10, a monoclonal antibody to PCNA The percentage of tumour cells with positive staining for PCNA was found to correlate well with histological grading Clinical follow-up data were available in 25 adults and showed no known deaths in 11 cases with a low proportion (< 14%) of positive cells Out of 14 cases with a high number (greater-than-or-equal-to 14%) of positive cells, seven patients are known to have died, two had metastases, and in a further two there have been multiple recurrences of tumour DNA flow cytometry was performed on 26 cases but this showed no correlation with PC10 staining or clinical outcome Staining with PC10 may be of particular value in the identification of patients at greatest risk of rapid tumour metastasis and early deathread more
Citations
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Journal ArticleDOI
Myofibromatosis in adults, glomangiopericytoma, and myopericytoma. A spectrum of tumors showing perivascular myoid differentiation
TL;DR: The study suggests that these three lesional groups comprise a histologic continuum of tumors that share clinical similarities and that, perhaps, are designated more appropriately as perivascular myomas.
Journal ArticleDOI
Immunohistological detection of tumour growth fraction (Ki-67 antigen) in formalin-fixed and routinely processed tissues.
Journal ArticleDOI
The assessment of cellular proliferation by immunohistochemistry: a review of currently available methods and their applications.
TL;DR: Immunohistochemical methods using antibodies to cell cycle-related antigens may be used as a means of assessing various aspects of proliferation in tissue, and have the important advantage of preserving the spatial orientation of proliferating cells in histological sections.
Journal ArticleDOI
Evaluation of cell proliferation in rat tissues with BrdU, PCNA, Ki-67(MIB-5) immunohistochemistry and in situ hybridization for histone mRNA.
TL;DR: Results suggest that both ISH for histone mRNA and IHC with MIB-5 are preferable techniques for assessment of cell proliferation in rat paraffin-embedded renewing tissues compared to PCNA IHC.
Journal ArticleDOI
The complexities of proliferating cell nuclear antigen.
D. McCORMICK,P.A. Hall +1 more
TL;DR: The last 2 years have seen a flood of reports in Histopathology and other pathology journals describing the application of antibodies that recognize proliferating cell nuclear antigen (PCNA) as a marker of cell proliferation in histological material, and current understanding of this player in the processes of DNA replication is clarified.
References
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Journal ArticleDOI
Method for analysis of cellular DNA content of paraffin-embedded pathological material using flow cytometry
TL;DR: There was a good correlation between the DNA histograms produced using this method and those obtained using unfixed tissue from the same tumor stained with ethidium bromide plus mithramycin.
Journal ArticleDOI
Proliferating cell nuclear antigen (PCNA) immunolocalization in paraffin sections: An index of cell proliferation with evidence of deregulated expression in some, neoplasms
Peter A. Hall,David A. Levison,A. L. Woods,C. C.-W. Yu,D. B. Kellock,J. A. Watkins,Diana M. Barnes,Cheryl Gillett,Richard Camplejohn,Robin Dover,N. H. Waseem,David P. Lane +11 more
TL;DR: Data suggest that in normal tissues and lymphoid neoplasms, PCNA immunolocalization can be used as an index of cell proliferation, however, in some forms of neoplasia, including breast and gastric cancer and in vitro cell lines, the simple relation between PCNA expression and cell proliferation is lost.
Journal ArticleDOI
Hemangiopericytoma: a vascular tumor featuring zimmermann's pericytes.
TL;DR: HemangIOPERICYTOMA A VASCULAR TUMOR FEATURing ZIMMERMANN's PericYTES is presented, demonstrating the importance of knowing the carrier and removal status of the immune system-derived Tournaisianutra.
Journal ArticleDOI
Existence of two populations of cyclin/proliferating cell nuclear antigen during the cell cycle: association with DNA replication sites.
R Bravo,H Macdonald-Bravo +1 more
TL;DR: It was shown that the staining patterns of the replicon clusters and their order of appearance throughout the S phase are identical to those observed for cyclin, demonstrating that cyclin is tightly associated to the sites of DNA replication and that it must have a fundamental role in DNA synthesis in eukaryotic cells.
Journal ArticleDOI
Hemangiopericytoma. An analysis of 106 cases.
Franz M. Enzinger,Bruce H. Smith +1 more
TL;DR: The close resemblance of hemangiopericytoma to richly vascular forms of fibrous histiocytomas and synovial sarcoma is emphasized, and the morphologic differences from other mesenchymal tumors showing a hemango-like vascular pattern are discussed.
Related Papers (5)
Monoclonal antibody analysis of the proliferating cell nuclear antigen (PCNA). Structural conservation and the detection of a nucleolar form.
N.H. Waseem,David P. Lane +1 more