Journal ArticleDOI
High pressure liquid chromatographic determination of theobromine and caffeine in cocoa and chocolate products.
TLDR
A method was developed for determining theobromine and caeffine in cocoa and chocolate products by high pressure liquid chromatography by using a reverse phase C18 column and a mobile phase of methanol-water-acetic acid.Abstract:
A method was developed for determining theobromine and caeffine in cocoa and chocolate products by high pressure liquid chromatography. After a simple hot water extraction, both theobromine and caffeine were separated by using a reverse phase C18 column and a mobile phase of methanol-water-acetic acid (20 + 79 + 1). Theobromine and caffeine were quantitated at 280 nm; average recoveries were 98.7 and 95.0%; and coefficients of variation were 2.31 and 3.91%, respectively.read more
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Journal ArticleDOI
Fermentation of cocoa beans: influence of microbial activities and polyphenol concentrations on the flavour of chocolate
TL;DR: In this paper, the same micro-organisms were involved in these heaps, carried out at different farms or in different seasons, heap temperatures and microbial metabolite concentrations were different.
Journal ArticleDOI
Influence of Turning and Environmental Contamination on the Dynamics of Populations of Lactic Acid and Acetic Acid Bacteria Involved in Spontaneous Cocoa Bean Heap Fermentation in Ghana
Nicholas Camu,Ángel F. González,Tom De Winter,Ann Van Schoor,Katrien De Bruyne,Peter Vandamme,Jemmy S. Takrama,Solomon K. Addo,Luc De Vuyst +8 more
TL;DR: The influence of turning and environmental contamination on six spontaneous cocoa bean heap fermentations performed in Ghana was studied through a multiphasic approach, encompassing both microbiological (culture-dependent and culture-independent techniques) and metabolite target analyses.
Journal ArticleDOI
HPLC Determination of Caffeine and Theobromine in Coffee, Tea, and Instant Hot Cocoa Mixes
Joan L. Blauch,Stanley M. Tarka +1 more
TL;DR: In this article, the methylxanthines were separated by high performance liquid chromatography using a reverse phase C18 column and a mobile phase of acetonitrile and water (8:92, v/v).
Journal ArticleDOI
Effects of short-term and long-term theobromine administration to male dogs.
TL;DR: While right atrial cardiomyopathy occurred in dogs given theobromine 100 or 150 mg/kg/day, a clear relationship between dose, plasma concentration, frequency, and severity of the lesion could not be determined.
Journal ArticleDOI
Cacao Residues in Ancient Maya Vessels from Rio Azul, Guatemala
TL;DR: In this article, chemical analyses on residues collected from ceramic vessels found in an Early Classic period Maya tomb revealed that certain of the residues contained theobromine and caffeine, a clear indication that the corresponding vessels once contained cacao in some form.
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