Journal ArticleDOI
High-resolution DNA melting analysis for simple and efficient molecular diagnostics
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TLDR
High-resolution DNA melting has several advantages over other genotyping and scanning methods, including an inexpensive closed tube format that is homogenous, accurate and rapid, and a good fit for personalized medicine as a rapid, inexpensive method to predict therapeutic response.Abstract:
High-resolution melting of DNA is a simple solution for genotyping, mutation scanning and sequence matching. The melting profile of a PCR product depends on its GC content, length, sequence and heterozygosity and is best monitored with saturating dyes that fluoresce in the presence of double-stranded DNA. Genotyping of most variants is possible by the melting temperature of the PCR products, while all variants can be genotyped with unlabeled probes. Mutation scanning and sequence matching depend on sequence differences that result in heteroduplexes that change the shape of the melting curve. High-resolution DNA melting has several advantages over other genotyping and scanning methods, including an inexpensive closed tube format that is homogenous, accurate and rapid. Owing to its simplicity and speed, the method is a good fit for personalized medicine as a rapid, inexpensive method to predict therapeutic response.read more
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Checklist of catfishes, recent and fossil (Osteichthyes: Siluriformes), and catalogue of siluriform primary types
TL;DR: A checklist of Recent and fossil catfishes (Order Siluriformes) is presented, summarizing taxonomic literature published through 2005, and one new name is proposed herein: Clariallabes teugelsi, as a replacement for Clarias (Allabenchelys) dumerili longibarbis David & Poll, 1937.
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The use of gold nanoparticles in diagnostics and detection
TL;DR: Methods that allow target molecules to be detected with the unaided eye are focused on because these, more than any other, harness the full range of properties that make GNPs unique.
Journal ArticleDOI
High-resolution dna melting analysis: advancements and limitations
TL;DR: This focus issue of Human Mutation includes a concise, timely review on high resolution melting, a comparison to denaturing gradient gel electrophoresis, integration with qPCR for copy number assessment, combined amplicon scanning and unlabeled probe genotyping from a single melting curve, and applications to the mitochondrial genome and to BRCA1.
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Molecular testing for BRAF mutations to inform melanoma treatment decisions: a move toward precision medicine.
TL;DR: A literature search was performed using MEDLINE/PubMed and scientific congress databases using the terms ‘BRAF,’ ‘mutation, and ‘cancer/tumor.’ These results were filtered to include diagnostic tests for determining BRAF mutation status as mentioned in this paper.
Journal ArticleDOI
High resolution melting applications for clinical laboratory medicine
TL;DR: High resolution PCR product melting is homogeneous, closed-tube, rapid (1-5 min), non-destructive and does not require covalently-labeled fluorescent probes, an ideal format for in-house testing, with minimal cost and time requirements for new assay development.
References
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TL;DR: The mobility shift analysis of single-stranded DNAs on neutral polyacrylamide gel electrophoresis to detect DNA polymorphisms was developed and SSCPs were found to be allelic variants of true Mendelian traits, and therefore they should be useful genetic markers.
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Journal ArticleDOI
High-Resolution Genotyping by Amplicon Melting Analysis Using LCGreen
TL;DR: High-resolution melting analysis of PCR products amplified in the presence of LCGreen can identify both heterozygous and homozygous sequence variants, and is a promising method for mutation screening.
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Diagnostic molecular microbiology : principles and applications
TL;DR: Principles of Diagnostic Molecular Microbiology Applications ofdiagnostic molecular Microbiology Section 1: Bacterial Pathogens Secton 2: Viral Pathogen Section 3: Fungal Pathogens Section 4: Parasitic Pathogens section 5: Novel Organisms Section 6: Antimicrobial Resistance Loci Section 7: Molecular Typing Methods Section 8: Methods
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