Patent
HLA D Typing assay
TLDR
In this article, an assay for HLA D phenotype where antigen-pulsed monocytes are contacted with antigen-specific T lymphocytes or T cell hybridomas was proposed.Abstract:
An assay for HLA D phenotype wherein antigen-pulsed monocytes are contacted with antigen-specific T lymphocytes or T cell hybridomas and the extent of selective binding between the pulsed monocytes and the T lymphocytes or T cell hybridomas determines HLA D phenotype of the monocyte. The assay is particularly useful for quickly typing donortissue prior to transplantation.read more
Citations
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Hla typing method and cdna probes used therein
TL;DR: In this paper, a restriction length polymorphism based HLA typing is carried out by digesting an individual's HLA DNA with a restriction endonuclease that produces a polymorphic digestion pattern with HLA, subjecting the digest to genomic blotting using a labeled cDNA hybridization probe that is complementary to an HLA sequence involved in the polymorphism.
Patent
Transgenic animals harboring app allele having swedish mutation
Lisa C. Mcconlogue,Jun Zhoa +1 more
TL;DR: In this paper, a transgene encoding an APP polypeptide comprising the Swedish mutation was proposed. But it was not suitable for transgenic non-human animals and transgenic mammalian cells.
Patent
Methods of screening for beta-amyloid peptide production inhibitors
TL;DR: In this article, the β-amyloid precursor protein (βAPP) is monitored by detecting the secretion of a soluble amino-terminal fragment or βAPP (ATF-βAPP).
Patent
Methods and compositions for monitoring cellular processing of beta-amyloid precursor protein
TL;DR: In this article, the β-amyloid precursor protein (βAPP) is monitored by detecting the secretion of a soluble bAPP fragment resulting from cleavage of βAPP at the amino-terminus of β-AMyloid peptide.
Patent
APP770 mutant in alzheimer's disease
John Hardy,Marie-Christine Chartier-Harlin,Alison Goate,Michael John Owen,Michael John Mullan +4 more
TL;DR: In this article, a model system of Alzheimer's disease comprises a DNA sequence encoding an amyloid precursor protein (APP) isoform or fragment that has an amino acid substitution.
References
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Patent
Hla typing method and cdna probes used therein
TL;DR: In this paper, a restriction length polymorphism based HLA typing is carried out by digesting an individual's HLA DNA with a restriction endonuclease that produces a polymorphic digestion pattern with HLA, subjecting the digest to genomic blotting using a labeled cDNA hybridization probe that is complementary to an HLA sequence involved in the polymorphism.
Journal ArticleDOI
Biochemical separation of a human B cell mitogenic factor
Abby L. Maizel,Chintaman G. Sahasrabuddhe,Shashikant Mehta,John W. Morgan,Lawrence B. Lachman,Richard J. Ford +5 more
TL;DR: During the isolation procedure the proliferation-stimulating activity of the column fractions was assayed concurrently on purified human T cells, purified human B cells, and murine thymocytes, and the fractions associated with B cell mitogenic activity induced B cell S-phase entry in a proportion of B lymphocytes in the absence of any detectable IgM secretion.
Journal ArticleDOI
Cloned cytotoxic and non-cytotoxic lymphocytes in mouse and man: their reactivities and a large cell surface membrane protein (LMP) differentiation marker system.
TL;DR: Cellular interactions within the immune system, which consists of an enormously complex series of positive and negative regulatory networks, can now be studied in a reductionist view involving the various contributing cell types, provided each of them can be cloned.
Patent
Automatic HLA typing apparatus
TL;DR: In this paper, a phase-contrast microscope was used for automatically typing HLA (Human Leukocyte Antigen) with high accuracy, where an optical image of a sample containing lymphocytes was formed by a phase contrast microscope, and the optical image was transformed into electrical picture signal through a image pick-up unit, which in turn is transformed into binary picture patterns for a predetermined area site in the image through a window circuit.
Patent
Process for determining the complement-dependent cytotoxicity mediated by anti-HLA antibodies by means of ATP determination and device for ATP determination
TL;DR: In this paper, a process for determining the complement-dependent cytotoxicity mediated by anti-HLA antibodies was proposed, which measured the loss of intracellular ATP after addition of complement to anti-HLA coated target cells.