Journal ArticleDOI
Hydrophilic interaction liquid chromatography coupled to mass spectrometry for the characterization of prebiotic galactooligosaccharides.
Oswaldo Hernández-Hernández,I. Calvillo,Rosa Lebrón-Aguilar,Francisco J. Moreno,María Luz Sanz +4 more
TLDR
Three different stationary phases (sulfoalkylbetaine zwitterionic, polyhydroxyethyl aspartamide and ethylene bridge hybrid (BEH) with trifunctionally bonded amide), operating at hydrophilic interaction liquid chromatographic (HILIC) mode, have been assayed and compared for the analysis of complex mixtures of galactooligosaccharides (GOS).About:
This article is published in Journal of Chromatography A.The article was published on 2012-01-13. It has received 55 citations till now. The article focuses on the topics: Hydrophilic interaction chromatography & Ammonium acetate.read more
Citations
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Journal ArticleDOI
Oligosaccharide analysis by mass spectrometry: A review of recent developments
TL;DR: This review covers developments in the application of mass spectrometry to the analysis of carbohydrates, with an emphasis on work that has occurred from January 2011 through October 2013.
Journal ArticleDOI
Recent developments in high-performance liquid chromatography stationary phases.
Journal ArticleDOI
Separation of carbohydrates using hydrophilic interaction liquid chromatography
TL;DR: A strategy was developed to rapidly evaluate chromatographic properties of hydrophilic interaction chromatography (HILIC) columns for separating carbohydrates, which indicated that buffer salt concentration and pH value in mobile phase was necessary to control the ionic interactions between ionic carbohydrates and HILIC columns.
Journal ArticleDOI
Analysis, structural characterization, and bioactivity of oligosaccharides derived from lactose.
TL;DR: The bioactive effects of galacto oligosaccharide MS fragmentation patterns, using different ionization sources and mass analyzers, as well as data on structural analysis by NMR spectroscopy are described.
Journal ArticleDOI
Characterization of goat colostrum oligosaccharides by nano-liquid chromatography on chip quadrupole time-of-flight mass spectrometry and hydrophilic interaction liquid chromatography-quadrupole mass spectrometry.
Andrea Martín-Ortiz,Jaime Salcedo,Daniela Barile,A. Bunyatratchata,Francisco J. Moreno,I. Martín-García,Alfonso Clemente,María Luz Sanz,Ana I. Ruiz-Matute +8 more
TL;DR: A hydrophilic interaction liquid chromatography coupled to mass spectrometry methodology was developed for the separation and quantitation of the main GCO, both acidic and neutral carbohydrates, and the combination of both techniques resulted to be useful to achieve a comprehensive characterization of GCO.
References
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Dietary Modulation of the Human Colonic Microbiota: Introducing the Concept of Prebiotics
TL;DR: By combining the rationale of pro- and prebiotics, the concept of synbiotics is proposed to characterize some colonic foods with interesting nutritional properties that make these compounds candidates for classification as health-enhancing functional food ingredients.
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Hydrophilic-interaction chromatography for the separation of peptides, nucleic acids and other polar compounds
TL;DR: Hydrophilic-interaction chromatography fractionations resemble those obtained through partitioning mechanisms, and the chromatography of DNA, in particular, resembles the partitioning observed with aqueous two-phase systems based on polyethylene glycol and dextran solutions.
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Mass spectrometry of oligosaccharides.
TL;DR: This review summarizes the state of knowledge for the mass spectrometric analysis of oligosaccharides with regard to neutral, sialylated, and sulfated compound classes to give the reader the background to make informed decisions to solve structure-activity relations in glycomics.
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Stationary and mobile phases in hydrophilic interaction chromatography: a review
TL;DR: This review deals with recent advances in the development of HILIC phase separation systems with special attention to the properties of stationary phases.
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Galacto‐Oligosaccharides: Production, Properties, Applications, and Significance as Prebiotics
TL;DR: This article focuses on GOS production from synthesis to purification processes, and several microbial GH, proposed for the synthesis of GOS, are hierarchized according to the referred performance indicators.