Identification of Cytokinins of Root Nodules of the Garden Pea, Pisum sativum L.
Kunihiko Syono,John G. Torrey +1 more
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Five cytokinin activities which induced soybean callus proliferation were detected in ethanol extracts of root nodules of the garden pea and the most active factors were identified as zeatin and its riboside on the basis of their mobility on thin layer chromatography in three solvent systems.Abstract:
Five cytokinin activities which induced soybean callus proliferation were detected in ethanol extracts of root nodules of the garden pea (Pisum sativum L., cv. Little Marvel). The most active factors among them were identified as zeatin and its riboside on the basis of their mobility on thin layer chromatography in three solvent systems. Smaller activities of zeatin ribotide, isopentenyladenine and its riboside were also detected. Cytokinin activity gradually decreased with the cultivation period, but no qualitative change in the active compounds was found.read more
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Production of gibberellins and indole-3-acetic acid by Rhizobium phaseoli in relation to nodulation of Phaseolus vulgaris roots.
TL;DR: The HPLC-immunoassay analyses showed that nodules and non-nodulated roots of Phaseolus vulgaris L. contained similar spectra of GAs to R. phaseoli culture media, indicating that Rhizobium does not make a major contribution to the GA content of the infected tissue.
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The role of phytohormones in plant-microbe symbioses
TL;DR: It is shown that the early nodulin (ENOD) genes can be used as indicators for the status of endogenous hormones in symbiotic root tissues and Transcripts for ENOD2 and ENOD40 genes are shown to accumulate in uninoculated, cytokinin-treated alfalfa roots, even in roots of the non-nodulating al falfa mutant MN1008.
Book ChapterDOI
Endogenous and exogenous influences on the regulation of lateral root formation
TL;DR: A lateral root is a side root that arises by cell division in the pericycle of the parent root that penetrates the cortex and its apical meristem is comparable with that of the apex of theparent root.
Journal ArticleDOI
The Rhizobium-Legume Symbiosis
TL;DR: Bisseling, van den Bos & van Kamman (1978) showed that under conditions where NH4NO3 repressed N2-ase activity in pea nodules, it did not affect synthesis of the enzyme, and proposed that the inhibition of N 2-ase in the nodule was indirect, being mediated by an inhibition of the synthesis of leg-haemoglobin.
References
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Journal ArticleDOI
Kinetin as trigger for mitosis in mature endomitotic plant cells
TL;DR: It has been concluded that Kinetin specifically triggers mitosis in mature pea root tissue cells which have undergone DNA- and chromosome-doubling by endomitotic reduplication before kinetin treatment.
Journal ArticleDOI
Initiation of Mitosis and Cell Division by Kinetin and Indoleacetic Acid in Excised Tobacco Pith Tissue
Journal ArticleDOI
Method for Growing Plants Aeroponically
TL;DR: A simple, inexpensive system for growing plants with their roots bathed in nutrient mist using a spinner from a home humidifier to propel nutrient solution into a plywood box atop which plants are supported on plastic light-fixture "egg crating".
Journal ArticleDOI
Cell division in relation to cytodifferentiation in cultured pea root segments
John G. Torrey,Donald E. Fosket +1 more
TL;DR: It was concluded that cytokinin, together with auxin, was essential for the initiation of DNA synthesis in the cortical cells, for their subsequent division, and finally for their specific cytodifferentiation.