Journal ArticleDOI
Immobilized cyclomaltodextrin glucanotransferase of an alkalophilic Bacillus sp. No. 38-2.
Takashi Kato,Koki Horikoshi +1 more
TLDR
The enzyme converted starch to cyclodextrins without significant loss of activity under the conditions of continuous reaction for about two weeks by using the column system and the optimum pH of immobilized enzyme was 9.0.Abstract:
Cyclomaltodextrin glucanotransferase [1,4-alpha-D-glucan-4-alpha-D-(1,4-alpha-D-glucano)-transferase (cyclizing), E.C.-2.4.1.19] of an alkalophilic Bacillus sp. No. 38-2 (ATCC 21783), which contains three types of enzymes (acid, neutral, and alkaline enzymes), was immobilized on synthetic adsorption resin. No distinguishing changes in pH or thermal stabilities of enzyme were observed due to the immobilization. Since acid-enzyme activity had disappeared, the optimum pH of immobilized enzyme was 9.0. Optimum temperature for the enzyme activity changed from 50 to 55 degrees C. The enzyme converted starch to cyclodextrins without significant loss of activity under the conditions of continuous reaction for about two weeks by using the column system (60 degrees C at pH 8.0). About 63% of soluble starch solution [4% (w/v)] was changed to cyclodextrins, as tested so far.read more
Citations
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Journal ArticleDOI
Bacterial cyclodextrin glucanotransferase
TL;DR: The present review attempts to summarize the reported data concerning the bacterial producers of CGTase, growth cultural conditions providing optimal enzyme biosynthesis in batches, repeated batch and continuous cultivation of free and immobilized cells, as well as some physicochemical and biochemical characteristics of the enzyme, C GTase immobilization, and enzyme structure.
Journal ArticleDOI
Characterization of Thermoanaerobacter cyclomaltodextrin glucanotransferase immobilized on glyoxyl-agarose
TL;DR: The immobilization of the Thermoanaerobacter cyclomaltodextrin glucanotransferase (CGTase) enzyme into cross-linked 6% agarose beads activated by high density of linear aldehyde groups (glyoxyl-agarose) that allow the establishment of multi-attachment enzyme-support bonds are presented.
Journal ArticleDOI
Immobilization of paenibacillus macerans nrrl b-3186 cyclodextrin glucosyltransferase and properties of the immobilized enzyme
TL;DR: Compared to the free enzyme, the immobilized form exhibited: a higher optimal reaction temperature and energy of activation, a higher K m and lower V max (maximal reaction rate), improved thermal stability and resistance to chemical denaturation.
Journal ArticleDOI
Synthesis of cyclodextrin glucosyl transferase by Bacillus cereus for the production of cyclodextrins.
TL;DR: A potent indigenous bacillus isolate identified asBacillus cereus (RJ-30) was found to produce Cyclodextrin Glucosyl Transferase (CGTase) extracellularly, and the influence of carbohydrate substrates has shown that enzyme synthesis is promoted by xylose and wheat bran extract in glucose medium.
Journal ArticleDOI
Screening for novel biocatalysts
TL;DR: The development of selective, non-random, goal-orientated screening techniques, methods of evaluating biocatalyst performance under operational conditions, and an approach that is more orientated towards commercially desirable goals are essential if these objectives are to be achieved.
References
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Journal ArticleDOI
Purification and Some Properties of Cyclodextrin Glycosyltransferase from a Strain of Bacillus Species
TL;DR: An ultracentrifugally homogeneous preparation of the cyclodextrin glycosyltransferase was obtained from the culture broth of a strain of Bacillus species (No. 5 strain) by a purification methods including adsorption on corn starch, ammonium sulfate precipitation, chromatography on DEAE-cellulose column, and gel-filtration on Sephadex G–100.
Journal ArticleDOI
Characterization and Some Cultural Conditions of a Cyclodextrin Glycosyltransferase-producing Alkalophilic Bacillus sp.
Nobuyuki Nakamura,Koki Horikoshi +1 more
TL;DR: In this paper, a cyclodextrin glycosyltransferase-producing bacterium was isolated from soil in a high alkaline pH medium containing 1% of Na2CO3.
Journal ArticleDOI
Purification and some properties of Bacillus macerans cycloamylose (cyclodextrin) glucanotransferase.
TL;DR: Bacillus macerans cycloamylose (cyclodextrin) glucanotransferase (EC 2.4.1.19) was purified by the technique of starch adsorption and DEAE-cellulose column chromatography, and then crystallized from an ammonium sulfate solution containing mM calcium chloride.
Journal ArticleDOI
Cyclodextrin-Glucanotransferase von Klebsiella pneumoniae
TL;DR: In this paper, the Cyclodextrin-Glucanotransferase-Grenzdextrin (GCG-GRS) was used in the treatment of Klebsiella pneumoniae.
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