Journal ArticleDOI
In vitro adventitious bud techniques for vegetative propagation and mutation breeding of potato (Solanum tuberosum L.). II. Significance for mutation breeding
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It was investigated what potentialities for mutation breeding of potato are offered by using adventitious sprouts that arise in vitro from leaf explants (rachis, petiole, leaflet-disc) after X-irradiation.Abstract:
It was investigated what potentialities for mutation breeding of potato are offered by using adventitious sprouts that arise in vitro from leaf explants (rachis, petiole, leaflet-disc) after X-irradiation. Mutation frequency and chimerism were studied in subterranean and aerial parts in three vegetative generations (vM1, vM2, vM3). Plants obtained from irradiated series produced a very high mutation frequency, a wide mutation spectrum and a very low rate of chimerism. Mutations were observed also in control series, especially in plants derived from the rachis and petiole explant group.read more
Citations
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Journal ArticleDOI
Somaclonal variation in plants: causes and detection methods
TL;DR: The possible causes, detection methods and desirability of variants are summarized, and examples of some useful variants generated as a result of somaclonal variation are outlined.
Book ChapterDOI
Somaclonal and gametoclonal variation
TL;DR: Somaclonal and gametoclonal variation are new tools for the geneticist and plant breeder that permit reduction in the time period for new variety development and that permit access to new classes of genetic variation as discussed by the authors.
Book ChapterDOI
Somaclonal Variation and Crop Improvement
Philip J. Larkin,W. R. Scowcroft +1 more
TL;DR: It is contention that the lateness of this realization was a consequence of the fact that so few tissue culturists were engaged in careful analysis of the regenerated plants and also failed to see cell culture manipulation in a genetic context.
Journal ArticleDOI
Phenotypic variation and ploidy level of plants regenerated from protoplasts of tetraploid potato (Solanum tuberosum L. cv. 'Bintje').
TL;DR: A wide range of phenotypic variation occurred among protoplast — derived plants of tetraploid potato cultivar ‘Bintje’ and the results suggest that the altered morphologies are caused predominantly by changes in ploidy levels.
Book ChapterDOI
Tissue Culture-Induced Variation and Crop Improvement
TL;DR: Only a synergistic and concerted effort by traditional breeders and plant biotechnologists can bring this technology to the potential level of efficiency and application that will result in enhanced breeding efforts and cultivars with improved value-added traits that can be grown profitably by producers.
References
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Application of mutation breeding methods in the improvement of vegetatively propagated crops
C. Broertjes,A. M. van Harten +1 more
Journal ArticleDOI
Vegetative propagation of Solanum tuberosum L. in vitro
S. Roest,G. S. Bokelmann +1 more
TL;DR: Preliminary experiments with the potato clone EM 52 are described, with results which demonstrate the possibility of the production of numerous adventitious shoots on explants of the rachis of a compund leaf cultivated in vitro.
Journal ArticleDOI
Adventitious bud formation on isolated leaves and its significance for mutation breeding
TL;DR: Many plant species can be stimulated to form adventitious buds on isolated leaves and in a number of cases it has been reported that only one cell was involved, resulting in a high frequency of solid, non-chimeral mutants and a wide mutation spectrum.
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