In-vitro induction of capacitation of fresh and frozen spermatozoa of the Siberian tiger (Panthera tigris).
A. P. Byers,A. G. Hunter,Ulysses S. Seal,G. A. Binczik,E. F. Graham,N. J. Reindl,Ronald Tilson +6 more
TLDR
Frozen semen experiments indicate that frozen-thawed tiger spermatozoa must be removed from the environment of the semen extender before capacitation can take place, and the freeze-thaw procedure results in a shortening of the required capacitation time.Abstract:
Electroejaculates from 5 tigers were split and half of each was assayed fresh while the remainder was frozen and thawed before being assayed. Preincubation time, temperature and removal of seminal plasma were evaluated for their effect on in-vitro capacitation. Ability of spermatozoa to penetrate oocytes, as measured by the zona-free hamster egg-sperm penetration assay (SPA), was used as verification of capacitation. Results of the experiments with fresh semen indicate that: (1) preincubation time affects the fertilizability of tiger spermatozoa with 2 h appearing optimal, (2) a preincubation temperature of 37 degrees C results in significantly higher penetration rates than does a 22 degrees C treatment, and (3) tiger seminal plasma does not appear to contain decapacitation factors, as has been reported for several other species. Frozen semen experiments indicate that (1) frozen-thawed tiger spermatozoa must be removed from the environment of the semen extender before capacitation can take place, and (2) the freeze-thaw procedure results in a shortening of the required capacitation time.read more
Citations
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Semen cryopreservation in domestic animals: a damaging and capacitating phenomenon.
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References
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The Use of Zona-Free Animal Ova as a Test-System for the Assessment of the Fertilizing Capacity of Human Spermatozoa
TL;DR: The results suggest that zona-free hamster ova can be substituted for human ova in the preliminary assessment of the fertilizing capacity of human spermatozoa.
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TL;DR: The data suggest that human spermatozoa fuse with the vitelline membrane of zona-free hamster eggs and decondense with varying efficiencies, and may have potential value as a diagnostic tool in evaluating human semenatozoal fertilizing capacity.