In vitro susceptibility testing of dermatophytes isolated in Goiania, Brazil, against five antifungal agents by broth microdilution method
Crystiane Rodrigues de Araujo,Karla Carvalho Miranda,Orionalda de Fátima Lisboa Fernandes,Ailton José Soares,Maria do Rosário Rodrigues Silva +4 more
TLDR
In this study, the microtiter plates were incubated at 28 masculineC allowing a reading of the minimal inhibitory concentration (MIC) after four days of incubation for Trichophyton mentagrophytes and five days for T. rubrum and Microsporum canis to be read.Abstract:
Atividades antifungicas de fluconazol, itraconazol, cetoconazol, terbinafina e griseofulvina foram testadas pelo metodo de microdiluicao em caldo contra 60 isolados de dermatofitos. Os resultados mostraram que todos os isolados produziram crescimento claramente detectavel a 28 oC e a concentracao inibitoria minima (CIM) foi determinada apos quatro dias de incubacao para Trichophyton mentagrophytes e cinco dias para T. rubrum e Microsporum canis. A maioria dos isolados teve um padrao uniforme de suscetibilidade para os agentes antifungicos testados. Baixos valores de CIM como 0,03 µg/mL foram encontrados para 33,3%, 31,6% e 15% dos isolados para itraconazol, cetoconazol e terbinafina, respectivamente.read more
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Preparation and evaluation of antifungal efficacy of griseofulvin loaded deformable membrane vesicles in optimized guinea pig model of Microsporum canis--dermatophytosis.
Nidhi Aggarwal,Shishu Goindi +1 more
TL;DR: The results indicated that the topical formulation of DMVs of griseofulvin could be utilized as an alternative to reduce the encumbrance of conventional oral formulations.
Journal ArticleDOI
Formulation, characterization and evaluation of an optimized microemulsion formulation of griseofulvin for topical application.
TL;DR: The main objective of the study was to develop a microemulsion (ME) formulation of griseofulvin for the treatment of dermatophytosis and it was observed to be non-sensitizing, histopathologically safe, and stable at 5±3°C, 25± 2°C and 40±2°C for a period of six months.
Journal ArticleDOI
Molecular Characterization and In Vitro Antifungal Susceptibility of 316 Clinical Isolates of Dermatophytes in Iran
Saham Ansari,Mohammad Taghi Hedayati,Kamiar Zomorodian,Keyvan Pakshir,Hamid Badali,Abdollah Rafiei,Mostafa Ravandeh,Seyedmojtaba Seyedmousavi,Seyedmojtaba Seyedmousavi,Seyedmojtaba Seyedmousavi +9 more
TL;DR: Analysis of data revealed a significant increase in the frequency of A.benhamiae, which definitely warrants further investigation to explore source of this infection in south of Iran, and terbinafine was the most effective antifungal against all isolates, in vitro.
Journal Article
Antifungal activity of some medicinal plants used in Jeddah, Saudi Arabia.
TL;DR: In this paper, methanol extract of lemon grass, lanta and nerium followed by their ethyl acetate extracts showed the highest activities against Trichophyton rubrum, respectively at a concentration of 100 µg ml −1, while aqueous extracts inhibited the growth of this fungus at the same concentration by 32-77%.
Journal ArticleDOI
An ancient plant Lawsonia inermis (henna): determination of in vitro antifungal activity against dermatophytes species.
G.S. Gozubuyuk,E. Aktas,N. Yigit +2 more
TL;DR: Henna paste showed the high antifungal activity against all dermatophytes species (20 to 50mm inhibition zone) and L. inermis (henna) was determined by agar diffusion method and henna was used as paste form.
References
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Reference method for broth dilution antifungal susceptibility testing of filamentous fungi : Approved standard
John H. Rex,Clinical +1 more
TL;DR: The Quality System Approach is intended to provide a guide to the development and use of such systems in the rapidly changing environment as to ensure their effectiveness and efficiency.
Journal ArticleDOI
Clinical Trichophyton rubrum Strain Exhibiting Primary Resistance to Terbinafine
Pranab K. Mukherjee,Steven D. Leidich,N. Isham,Ingrid Leitner,Neil S. Ryder,Mahmoud A. Ghannoum +5 more
TL;DR: The terbinafine-resistant isolates exhibited normal susceptibilities to clinically available antimycotics including itraconazole, fluconazoles, and griseofulvin, however, these isolates were fully cross resistant to several other known squalene epoxidase inhibitors, suggesting a target-specific mechanism of resistance.
Journal ArticleDOI
Antifungal susceptibility testing of dermatophytes: establishing a medium for inducing conidial growth and evaluation of susceptibility of clinical isolates.
TL;DR: This study used the oatmeal cereal agar for conidial formation along with the optimal conditions for dermatophyte susceptibility testing proposed by Norris et al. and determined the antifungal susceptibilities of 217 dermatophytes to fluconazole, griseofulvin, itraconazoles, and terbinafine.
Journal ArticleDOI
In vitro activities of 10 antifungal drugs against 508 dermatophyte strains
Belkys Fernández-Torres,A Carrillo,E. Martín,A. Del Palacio,Mary Moore,Anastasio Valverde,M. L. Serrano,Josep Guarro +7 more
TL;DR: In general, with the exception of fluconazole and G-1, all antifungals were shown to be highly effective.
Journal ArticleDOI
Collaborative Evaluation of Optimal Antifungal Susceptibility Testing Conditions for Dermatophytes
Belkys Fernández-Torres,F. J. Cabañes,Alfonso Javier Carrillo-Muñoz,Alexandre Esteban,Isabel Inza,L. Abarca,Josep Guarro +6 more
TL;DR: A multicenter study was conducted to define the most suitable testing conditions for antifungal susceptibility of dermatophytes and optimal testing conditions identified were an inoculum of 104 CFU/ml, a temperature of incubation of 28°C, an incubation period of 7 days, and MIC-0.