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Reference method for broth dilution antifungal susceptibility testing of filamentous fungi : Approved standard

John H. Rex, +1 more
TLDR
The Quality System Approach is intended to provide a guide to the development and use of such systems in the rapidly changing environment as to ensure their effectiveness and efficiency.
Abstract
...................................................................................................................................................i Committee Membership..........................................................................................................................v Active Membership.............................................................................................................................. vii Foreword...............................................................................................................................................xv The Quality System Approach.............................................................................................................xvi

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Methods for in vitro evaluating antimicrobial activity: A review

TL;DR: An exhaustive list of in vitro antimicrobial susceptibility testing methods and detailed information on their advantages and limitations are reported.
Journal ArticleDOI

Diagnosis and management of Aspergillus diseases: executive summary of the 2017 ESCMID-ECMM-ERS guideline

Andrew J. Ullmann, +58 more
TL;DR: Treatment duration for aspergillosis is strongly recommended based on clinical improvement, degree of immunosuppression and response on imaging, and in refractory disease, where a personalized approach considering reversal of predisposing factors, switching drug class and surgical intervention is also strongly recommended.
Journal ArticleDOI

Antifungal drug resistance: mechanisms, epidemiology, and consequences for treatment.

TL;DR: Standardized methods for reliable in vitro antifungal susceptibility testing are now available from the Clinical and Laboratory Standards Institute (CLSI) in the United States and the European Committee on Antimicrobial Susceptibility Testing (EUCAST) in Europe.
Journal ArticleDOI

Emergence of azole resistance in Aspergillus fumigatus and spread of a single resistance mechanism.

TL;DR: The presence of a dominant resistance mechanism in clinical isolates suggests that isolates with this mechanism are spreading in the authors' environment, and might be more prevalent than currently acknowledged.
References
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Journal ArticleDOI

Itraconazole resistance in Aspergillus fumigatus.

TL;DR: Invasive aspergillosis is an increasingly frequent opportunistic infection in immunocompromised patients and only two agents, amphotericin B and itraconazole, are licensed for therapy, and at least two mechanisms of resistance are responsible.
Journal ArticleDOI

Comparative and collaborative evaluation of standardization of antifungal susceptibility testing for filamentous fungi.

TL;DR: The results of the study imply that it may be possible to develop a reference method for antifungal susceptibility testing of filamentous fungi.
Journal ArticleDOI

Correlation between in-vitro susceptibility testing to itraconazole and in-vivo outcome of Aspergillus fumigatus infection.

TL;DR: With careful selection and standardization of test conditions it is possible to generate reproducible in-vitro susceptibility data for Aspergillus spp.
Journal ArticleDOI

In-vitro testing of susceptibility to amphotericin B is a reliable predictor of clinical outcome in invasive aspergillosis.

TL;DR: The outcome of aspergillus infection depends on the in-vitro susceptibility of the isolates to amphotericin B, and survival was poor in patients with isolates resistant to amph esotericin B and good in those with amphoteric in B-susceptible specimens.
Journal ArticleDOI

Quality Control Limits for Broth Microdilution Susceptibility Tests of Ten Antifungal Agents

TL;DR: An eight-laboratory collaborative study was carried out to document reproducibility of tests of Candida parapsilosis ATCC 22019 and Candida krusei ATCC 6258 by the NCCLS method.
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