Indole Alkaloids from the Leaves of Rauvolfia yunnanensis

TLDR: Wang et al. as mentioned in this paper used column chromatography (silica, alumina and Sephadex LH-20 gel) methods to identify caberine (1), 19-ethoxyl-demethyl-Delta'-17-acetylajmaline(2), vellosimine(3), beta-yohimbine(4), yohimbines(5), vinorine(6), picrinine(7), nareline (8), akuammicine(9), strictamine(10), reserpine(

Abstract: Rauvolfia yuananensis Tsiang are commonly used as a folklore herb to treat hypertension, snake bites, and insanity in the southwest of China, and an important industrial raw material of reserpine and vertical. But the wild resources of it reduced gradually in recent years, thus we have introduced and cultivated it to the arid-hot valley region of Yunnan Province, China, and investigated the chemical constituents variety of it for the sustainable utilization. The dried leaves powder (8.0 kg) of R. yunnanensis was extracted with 95% (volume fraction) EtOH(40 L x5, 8 h each), and led to the isolation of eleven indole alkaloids by column chromatography (silica, alumina and Sephadex LH-20 gel) methods. Their structures were identified as caberine (1), 19-ethoxyl-demethyl-Delta'-17-acetylajmaline(2), vellosimine(3), beta-yohimbine(4), yohimbine(5), vinorine(6), picrinine(7), nareline (8), akuammicine(9), strictamine(10), reserpine(11) by spectral analysis (UV, IR, MS and 1 D, 2D NMR), respectively. Among them, compound 2 was a new indole alkaloids, compounds 1, 8 and 9 were firstly isolated from this plant.