Influence of growth temperature on glucose metabolism of a psychotrophic strain of Bacillus cereus.
B H Chung,R Y Cannon,R C Smith +2 more
TLDR
Radiorespirometric analyses revealed that vegetative cells of B. cereus metabolized glucose by simultaneous operation of the Embden-Meyerhof-Parnas pathway and the pentose phosphate pathway, and the extracellular products resulting from the metabolism of glucose decreased as the growth temperature was lowered.Abstract:
The influence of temperature on glucose metabolism of a psychotrophic strain of Bacillus cereus was investigated. The pH of the growth medium and spore-forming frequencies of B. cereus varied when grown at 32, 20, or 7 C. Radiorespirometric analyses revealed that vegetative cells of B. cereus metabolized glucose by simultaneous operation of the Embden-Meyerhof-Parnas pathway and the pentose phosphate pathway. As the growth temperature decreased, glucose was metabolized with increased participation of the pentose phosphate pathway. The shift of cells grown at a higher temperature to a lower temperature increased the relative participation of the pentose phosphate pathway, whereas the shift of cells grown at low temperatures to a higher temperature had the opposite effect. Cells of late logarithmic phase grown at 20 and 7 C oxidized acetate by the tricarboxylic acid cycle reaction. However, cells grown at 32 C failed to oxidize acetate to CO2 to any appreciable extent. The extracellular products resulting from the metabolism of glucose decreased as the growth temperature was lowered. Organic acids were the major extracellular products of cultures grown at 32 and 20 C. Acetic acid, lactic acid, and pyruvic acid together accounted for 86.1 and 78.9% of extracellular radioactivity, respectively, at the two temperatures. The relative ratio of these three acids varied between the temperatures. Little or no acid accumulated at 7 C.read more
Citations
More filters
Journal ArticleDOI
Effect of temperature on metabolic activity of intact microbial communities: Evidence for altered metabolic pathway activity but not for increased maintenance respiration and reduced carbon use efficiency
Paul Dijkstra,Scott C. Thomas,Paul L Heinrich,George W. Koch,Egbert Schwartz,Bruce A. Hungate +5 more
TL;DR: In response to temperature, communities behave biochemically similarly to single species and the understanding of temperature effects on CUE, energy production and use for maintenance and growth processes is still incomplete.
Journal ArticleDOI
The adaptive response of bacterial food-borne pathogens in the environment, host and food: Implications for food safety.
Avelino Alvarez-Ordóñez,Véronique Broussolle,Pierre Colin,Christophe Nguyen-The,Miguel Prieto +4 more
TL;DR: Certain strategies used by food-borne bacteria to respond to particular stresses (acid, cold stress, extreme pressure) in a permanent or transient manner are reviewed and the implications that such adaptive responses pose for food safety are discussed.
Journal ArticleDOI
Adaptation of Bacillus cereus, an ubiquitous worldwide-distributed foodborne pathogen, to a changing environment
Frédéric Carlin,Frédéric Carlin,Julien Brillard,Julien Brillard,Véronique Broussolle,Véronique Broussolle,Thierry Clavel,Thierry Clavel,Catherine Duport,Catherine Duport,Michel Jobin,Michel Jobin,Marie-Hélène Guinebretière,Marie-Hélène Guinebretière,Sandrine Auger,Alexei Sorokine,Christophe Nguyen-The,Christophe Nguyen-The +17 more
TL;DR: The Bacillus cereus group (or B. cereus sensu lato) displays a wide diversity of strains recently distributed in seven major phylogenetic groups and may represent an interesting model to study the impact of climate change on foodborne pathogens.
Journal ArticleDOI
Characterization of aerobic and anaerobic vegetative growth of the food-borne pathogen Bacillus cereus F4430/73 strain
TL;DR: Uncontrolled batch cultures on several defined media showed that B. cereus strains had high amino acid or pyruvate requirements for anaerobic fermentative growth, and growth performance was considerably improved by maintaining the pH of the culture medium near neutrality.
References
More filters
Journal ArticleDOI
Effect of temperature on the composition of fatty acids in escherichia coli.
Allen G. Marr,John L. Ingraham +1 more
TL;DR: Variations in the temperature of growth and in the composition of the medium alter the proportions of individual fatty acids in the lipids of Escherichia coli.
Journal ArticleDOI
An o-Toluidine Method for Body-Fluid Glucose Determination
TL;DR: o-Toluidine, 6% (v/v) in glacial acetic acid, is used to determine glucose in biologic material after deproteinization with 3% trichloracetic acid and follows Beer's Law over a very wide range of concentrations.
Book ChapterDOI
The Biochemistry of the Bacterial Endospore
TL;DR: This chapter focuses on the present chemical knowledge of the mature spore along with theBiochemistry of its formation, and presents definitions and cytological changes during sporogenesis that help in assisting the understanding of the biochemistry of the spore.
Related Papers (5)
Effect of pH on the growth and glucose metabolism of Neisseria gonorrhoeae.
S A Morse,B H Hebeler +1 more
Energetics of Bacillus stearothermophilus growth: molar growth yield and temperature effects on growth efficiency.
T P Coultate,T K Sundaram +1 more
Carbohydrate metabolism in Rhiodopseudomonas capsulata: Enzyme titers, glucose metabolism, and polyglucose polymer synthesis
Leon Eidels,Jack Preiss +1 more