Open Access
Insecticidal crystal proteins of Bacilllus thuringiensis
H. Höfte,H.R. Whiteley +1 more
TLDR
Studies on the biochemical mechanisms of toxicity suggest that B. thuringiensis crystal proteins induce the formation of pores in membranes of susceptible cells, and these approaches are potentially powerful strategies for the protection of agriculturally important crops against insect damage.Abstract:
characterized by its ability to produce crystalline inclusions during spor-ulation. TIhese inclusions consist of proteins exhibiting a highly specific insecticidal activity (reviewed in references 4 and 97). Many B. thuriuigiensis strains with different insect host spectra have been identified (9). TIhey ar-e classified into different serotypes or subspecies based on their flagellar antigens. Most strains are active against larvae of certain members of the Lepidoptera, but some show toxicity against dipterian (reviewed in refer-ence 22) or coleopteran (53) species. For several cryst.al-producing strains.read more
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Bacillus thuringiensis and Its Pesticidal Crystal Proteins
E. Schnepf,Neil Crickmore,J. Van Rie,Didier Lereclus,J. Baum,Jerald S. Feitelson,Daniel R. Zeigler,Donald H. Dean +7 more
TL;DR: Researchers are reporting promising results in engineering more-useful toxins and formulations, in creating transgenic plants that express pesticidal activity, and in constructing integrated management strategies to insure that these products are utilized with maximum efficiency and benefit.
Journal ArticleDOI
Biology, Ecology, and Management of the Diamondback Moth
N. S. Talekar,Anthony M. Shelton +1 more
TL;DR: Lack of parasitoids in a particular area may have occurred because the diamondback moth is better able than its natural-enemy complex to become established in newly planted cmcifers.
Journal ArticleDOI
Revision of the Nomenclature for the Bacillus thuringiensis Pesticidal Crystal Proteins
Neil Crickmore,Daniel R. Zeigler,Jerald S. Feitelson,E. Schnepf,J. Van Rie,Didier Lereclus,J. Baum,Donald H. Dean +7 more
TL;DR: A new nomenclature, based on hierarchical clustering using amino acid sequence identity, is proposed, consisting of 133 crystal proteins comprising 24 primary ranks are systematically arranged.
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Field Performance of Elite Transgenic Maize Plants Expressing an Insecticidal Protein Derived from Bacillus thuringiensis
Michael G. Koziel,Gary L. Beland,Cindy Grimmer Bowman,Nadine Carozzi,Rebecca W. Crenshaw,Lyle Crossland,John Dawson,Nalini Desai,Martha Hill,Sue H. Kadwell,Karen Launis,Kelly S. Lewis,Daryl Maddox,Kathryn McPherson,Moez R. Meghji,Ellis J. Merlin,Richard Rhodes,Gregory W. Warren,Martha S. Wright,Stephen V. Evola +19 more
TL;DR: In this article, a synthetic gene encoding a truncated version of the CryIA(b) protein derived from Bacillus thuringiensis was introduced into immature embryos of an elite line of maize using microprojectile bombardment.
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The mode of action of Bacillus thuringiensis endotoxins.
TL;DR: The use of synthetic organic insecticides developed during the last half of this century may pose risks to human health and can cause environmental damage, Consequently, interest has developed in using alternative strategies for insect pest management, including Bacillus thuringiensis toxins as insecticides.
References
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Journal ArticleDOI
Analysis of membrane and surface protein sequences with the hydrophobic moment plot.
TL;DR: An algorithm has been developed which identifies alpha-helices involved in the interactions of membrane proteins with lipid bilayers and which distinguishes them from helices in soluble proteins, and suggests four transmembrane helices and a surface-seeking helix in fragment B, the moiety known to have trans Membrane function.
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Transgenic plants protected from insect attack
Mark Vaeck,Arlette Reynaerts,Herman Höfte,Stefan Jansens,Marc De Beuckeleer,Caroline Dean,Caroline Dean,Marc Zabeau,Marc Van Montagu,Jan Leemans +9 more
TL;DR: The Gram-positive bacterium Bacillus thuringiensis produces proteins which are specifically toxic to a variety of insect species, and modified genes have been derived from bt2, a toxin gene cloned from one Bacillus strain.
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Stable Transformation of Soybean (Glycine Max) by Particle Acceleration
TL;DR: Experiments using this protocol have resulted in mature RO and R1 plants expressing the introduced gene(s), regarded as a major step in the practical application of genetic engineering to the useful modification of food-crop species.
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Insect Tolerant Transgenic Tomato Plants
David A. Fischhoff,Katherine S. Bowdish,Katherine S. Bowdish,Frederick J. Perlak,Pamela G. Marrone,Sheila McCormick,Sheila McCormick,Jeanne G. Niedermeyer,Duff A. Dean,Kuniko Kusano-Kretzmer,Ernest J. Mayer,Dean E. Rochester,Stephen G. Rogers,Robert T. Fraley +13 more
TL;DR: The structure of an insect control protein gene from Bacillus thuringiensis var.
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Specificity of Bacillus thuringiensis delta-endotoxins is correlated with the presence of high-affinity binding sites in the brush border membrane of target insect midguts
TL;DR: The correlation between toxicity and specific binding is further strengthened by competition studies, as toxins active against dipteran or coleopteran larvae do not compete, and B. thuringiensis delta-endotoxins active against M. sexta compete for binding of 125I-labeled Bt2-toxin to M. brassicae vesicles.