Journal ArticleDOI
Stable Transformation of Soybean (Glycine Max) by Particle Acceleration
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TLDR
Experiments using this protocol have resulted in mature RO and R1 plants expressing the introduced gene(s), regarded as a major step in the practical application of genetic engineering to the useful modification of food-crop species.Abstract:
We have used particle acceleration by electric discharge to introduce DNA-coated gold particles into meristems of immature soybean seeds. Approximately 2 percent of shoots derived from these meristems via organogenesis were chimeric for expression of the introduced gene. Experiments using this protocol have resulted in mature RO and R1 plants expressing the introduced gene(s). We regard this work as a major step in the practical application of genetic engineering to the useful modification of food-crop species.read more
Citations
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Journal ArticleDOI
Insecticidal crystal proteins of Bacillus thuringiensis.
Herman Höfte,H R Whiteley +1 more
TL;DR: In this article, a classification for crystal protein genes of Bacillus thuringiensis is presented, based on the insecticidal spectra and the amino acid sequences of the encoded proteins.
Insecticidal crystal proteins of Bacilllus thuringiensis
H. Höfte,H.R. Whiteley +1 more
TL;DR: Studies on the biochemical mechanisms of toxicity suggest that B. thuringiensis crystal proteins induce the formation of pores in membranes of susceptible cells, and these approaches are potentially powerful strategies for the protection of agriculturally important crops against insect damage.
Journal ArticleDOI
Transformation of Maize Cells and Regeneration of Fertile Transgenic Plants.
W. J. Gordon-Kamm,T. M. Spencer,Mary L. Mangano,Thomas R. Adams,Richard J. Daines,William G. Start,James V. O'brien,Sheryl A. Chambers,W. R. Adams,Nancy Willetts,Thomas B. Rice,Catherine J. Mackey,R. W. Krueger,Albert P. Kausch,Peggy G. Lemaux +14 more
TL;DR: A reproducible system for the generation of fertile, transgenic maize plants has been developed and activity of the enzyme phosphinothricin acetyltransferase (PAT) encoded by bar were confirmed in all bialaphos-resistant callus lines.
Book ChapterDOI
Optimizing the biolistic process for different biological applications.
TL;DR: The technology has currently reached the point at which it can be readily and reliably used for a wide range of applications, and new applications can be optimized fairly readily.
Journal ArticleDOI
The Rx Gene from Potato Controls Separate Virus Resistance and Cell Death Responses
TL;DR: It is shown in both Nicotiana spp and potato that Rx has the potential to initiate a cell death response but that extreme resistance is separate and epistatic to necrosis, indicating that cell death and pathogen arrest are separate disease resistance responses in plants.
References
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Journal ArticleDOI
Detection of specific sequences among DNA fragments separated by gel electrophoresis.
TL;DR: This paper describes a method of transferring fragments of DNA from agarose gels to cellulose nitrate filters that can be hybridized to radioactive RNA and hybrids detected by radioautography or fluorography.
Journal ArticleDOI
GUS fusions: beta‐glucuronidase as a sensitive and versatile gene fusion marker in higher plants.
TL;DR: GUS is very stable, and tissue extracts continue to show high levels of GUS activity after prolonged storage, and Histochemical analysis has been used to demonstrate the localization of gene activity in cells and tissues of transformed plants.
Journal ArticleDOI
Genomic sequencing
George M. Church,Walter Gilbert +1 more
TL;DR: The genomic sequencing procedures are applicable to the analysis of genetic polymorphisms, DNA methylation at deoxycytidines, and nucleic acid-protein interactions at single nucleotide resolution.
Journal ArticleDOI
Assaying chimeric genes in plants: The GUS gene fusion system
TL;DR: Gene fusions can be defined its DNA constructions that result in the coding sequences from one gene (r@o,ter) being transcribed and/or translated under the direction of the controlling sequences of another gene (cmltrr).
Journal ArticleDOI
High-velocity microprojectiles for delivering nucleic acids into living cells
TL;DR: In this article, small tungsten particles (microprojectiles) are used to carry RNA or DNA into epidermal tissue of onion and these molecules were subsequently expressed genetically.
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