Journal ArticleDOI
Insight on biochemical characteristics of thermotolerant amylase isolated from extremophile bacteria Bacillus vallismortis TD6 (HQ992818)
Chandrasekaran Suganthi,Anbazhagan Mageswari,S. Karthikeyan,Kodiveri Muthukaliannan Gothandam +3 more
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TLDR
Halotolerant bacterium Bacillus vallismortis was isolated from saltern sediments in India, and produced significantly high levels extracellular amylase, which enables for easily adaptable setup of large scale production of the enzyme for use in detergent formulations.Abstract:
Halotolerant bacterium Bacillus vallismortis (HQ992818) was isolated from saltern sediments in India, and produced significantly high levels extracellular amylase. A detailed investigation on the culture conditions including period of incubation, media pH, and inoculum size in addition to different sources of carbon and nitrogen, metal ions, NaCl, and amino acids was carried out for optimized production. Maximum amylase production (62 U/mL) was attained after 26 h of incubation. The optimized conditions for maximal production of amylase were found to be 1% NaCl, pH 8, temp 37°C, 1% starch, 1% sodium nitrate, phenyl alanine (0.01%) and calcium chloride (10 mM). The biochemical characteristics of the extracellular amylase were studied with respect to change in temperature, pH and metal ions. The enzyme was found to be optimally active in the temperature range of 40–70°C and pH 8. Activation of the enzyme by Ca2+ (135%), Fe2+ (113%) and Mg2+ (109%) occurred at 5 mM concentration and strongly inhibited by Hg2+, Zn2+ and Mn2+ occurred at 10 mM. Significant compatibility of the enzyme with the commercial laundry detergents and the results of washing performance test confirmed its effectiveness. Available data on the optimized culture conditions enables for easily adaptable setup of large scale production of the enzyme for use in detergent formulations.read more
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Development of thermostable amylase enzyme from Bacillus cereus for potential antibiofilm activity.
Ramalingam Vaikundamoorthy,Rajaram Rajendran,Ananth Selvaraju,Kaviyarasan Moorthy,Santhanam Perumal +4 more
TL;DR: Findings suggested that marine bacterial derived amylase enzyme could be developed as potential antibiofilm agent.
Journal ArticleDOI
Random mutagenesis of super Koji (Aspergillus oryzae): improvement in production and thermal stability of α-amylases for maltose syrup production.
Bushra Aleem,Bushra Aleem,Muhammad Hamid Rashid,Muhammad Hamid Rashid,Neelam Zeb,Anam Saqib,Ayesha Ihsan,Mazhar Iqbal,Hazrat Ali +8 more
TL;DR: It was concluded that the developed five step screening process has great potential to generate potent mutants for the hyper production of thermostable enzymes through γ-rays mediated physical mutagenesis and has immense potential for application in saccharification process for maltose syrup production.
Journal ArticleDOI
Efficient two-step lactic acid production from cassava biomass using thermostable enzyme cocktail and lactic acid bacteria: insights from hydrolysis optimization and proteomics analysis
Anamika Sharma,Kumar Pranaw,Surender Singh,Surender Singh,Sunil Kumar Khare,Anuj K. Chandel,Pawan Kumar Singh Nain,Lata Nain +7 more
TL;DR: The study proved the potential renewable source of cassava biomass as a source for fermentable sugars that can be fermented to lactic acid with high yield and hopes this cost-effective and environmental-friendly bioprocess can be upscaled for industrial lactic Acid production.
Journal ArticleDOI
Statistical Optimization of Thermostable α-Amylase Production by a Newly Isolated Rhizopus oryzae Strain FSIS4 Using Decommissioned Dates
Amel Ait Kaki El-Hadef El-Okki,Mohammed Gagaoua,Leila Bennamoun,Shahrazed Djekrif,Kahina Hafid,Mohamed El-Hadef El-Okki,Zahia Meraihi +6 more
TL;DR: In this paper, a new Rhizopus oryzae strain FSIS4 isolated from wheat seed cultivated in Algerian Sahara was evaluated for the production of a thermostable α-amylase enzyme.
Journal ArticleDOI
Characterization of moderately thermostable α-amylase-producing Bacillus licheniformis from decaying potatoes and sweet potatoes.
TL;DR: In this article, the authors used Gram staining, spore staining and motility testing under aerobic conditions to identify local strains of α-amylase producing Bacillus licheniformis.
References
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Microbial α-amylases: a biotechnological perspective
TL;DR: This review focuses on the microbial amylases and their application with a biotechnological perspective and α-Amylase holds the maximum market share of enzyme sales with its major application in the starch industry as well as its well-known usage in bakery.
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Enzymatic properties of a novel thermostable, thermophilic, alkaline and chelator resistant amylase from an alkaliphilic Bacillus sp. isolate ANT-6
TL;DR: In this paper, a thermostable α-amylase producing Bacillus sp. ANT-6 was isolated from soil samples and the enzyme showed optimum activity at pH 10.5 and 80.5.
Journal ArticleDOI
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TL;DR: Three genera and five species of halophilic methylotrophic methanogens have been reported, and a bloom of phototrophic bacteria in the marine salterns of Salins-de-Giraud, located on the Mediterranean French coast in the Rhone Delta, is also described.